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Cold Spring Harbor Protocols, 2018
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Pradeep D. Uchil +2 more
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Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Pradeep D. Uchil +2 more
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Microbial Transformation and Transfection
Annual Review of Microbiology, 1966FORMATION OF COMPETENCE STATE 375 Physiological state of culture.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 375 Evidence for wall changes accompanying competence . . . . . . . . . . . . . . . . . . . .. 377 EVIDENCE FOR A DNA RECEPTOR OR COMPETENCE "FACTOR". 378 CHARACTERISTICS OF BIOLOGICALLY ACTIVE DNA.
A H Evans, J Spizizen, B E Reilly
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Transfection of Lymphoid Cells
Current Protocols in Immunology, 1994AbstractUsing available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more ...
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2003
Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Neuroscience and Behavioral Physiology, 2010
Transfection is a method of transforming cells based on the introduction into living cells of plasmids encoding a particular protein or RNA. This review describes the main methods of transfection and considers their advantages and disadvantages. Most attention is paid to lentivirus transduction as one of the most efficient methods for transforming ...
A. P. Bol’shakov, S. V. Salozhin
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Transfection is a method of transforming cells based on the introduction into living cells of plasmids encoding a particular protein or RNA. This review describes the main methods of transfection and considers their advantages and disadvantages. Most attention is paid to lentivirus transduction as one of the most efficient methods for transforming ...
A. P. Bol’shakov, S. V. Salozhin
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Transfection of Plasmodium falciparum
2012Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.
Melanie Rug, Alexander G. Maier
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Transfection microarray™ and the applications
Molecular BioSystems, 2009Microarray transfection has been extensively studied for high-throughput functional analysis of mammalian cells. However, control of efficiency and reproducibility are the critical issues for practical use. By using solid-phase transfection accelerators and nano-scaffold, we provide a highly efficient and reproducible microarray-transfection device ...
Jun Miyake +3 more
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Cationic Lipids for Transfection
Current Medicinal Chemistry, 2003Among other strategies, the use of cationic lipids as autoassembling vehicles for non viral DNA transfection has received considerable attention. An exponentially growing litterature has been published on this topic (over 700 hits for the past decade, including 400 in the last two years). The present review focuses on the main present strategies aiming
Michel Bessodes +4 more
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Transient transfection of DT40
2006Transient transfection of cell lines can be extremely useful in a number of applications. However, until recently, obtaining useful and reproducible percentages of transiently expressing DT40 was almost impossible. Standard techniques such as electroporation usually give low transfection efficiencies (can be up to 40%, but ususally less than 5%) while ...
Julian E. Sale, Roger Franklin
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1973
Hershey and Chase demonstrated in 1952 that DNA is the essential component of a phage particle which enters an E. coli cell upon infection with T2. DNA alone initiates and directs the two major reactions in phage infection: DNA replication, and synthesis of phage-specific proteins. Confirming this role of nucleic acids in the infective process, nucleic
Thomas A. Trautner, H. Ch. Spatz
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Hershey and Chase demonstrated in 1952 that DNA is the essential component of a phage particle which enters an E. coli cell upon infection with T2. DNA alone initiates and directs the two major reactions in phage infection: DNA replication, and synthesis of phage-specific proteins. Confirming this role of nucleic acids in the infective process, nucleic
Thomas A. Trautner, H. Ch. Spatz
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