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Liposome-Mediated Transfection
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology. Cationic liposomes are the most widely used class of carrier for transfection and a number of products are available commercially: Lipofectin (a 1:1 mixture of DOTMA [N-[1(2,3-dioleoyloxy)propyl]-N,N,N ...
David A, Dean, Joshua Z, Gasiorowski
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Liposome‐Mediated Transfection
Current Protocols in Immunology, 1992AbstractUsing liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the describes a transient expression system while an involves stable transformation and expression of DNA integrated into the genome of the transfected cell.
Michael, Whitt +2 more
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In vivo transfection microsystems
The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 2005Transfection is currently used to insert molecules into cells. In vivo transfection is mainly performed via viral or chemical transfection. However, electrical transfection is known to be a more efficient way to insert drugs into cells without side effects.
F, Rivera +4 more
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Dendrimers in gene transfection
Biochemistry (Moscow), 2009Dendrimers are a new class of nanocomposite materials. They are branching polymers whose structure is formed by monomeric subunit branches diverging to all sides from a central nucleus. The type of nucleus, attached monomers, and functional groups can be chosen during synthesis, which produces dendrimers of definite size, shape, density, polarity ...
D G, Shcharbin +2 more
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Biolistic Transfection of Neurons
Science's STKE, 2000One method used to study gene function is through the manipulation of gene expression by transfecting cells with DNA constructs designed to overexpress or knock out particular proteins. Unfortunately, transfection of cells and tissues remains a rate-limiting step for molecular studies in many fields, especially neurobiology.
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Keratinocyte Transient Transfections
2004The goal of this chapter is to give the reader a concise and easy-to-follow guide to proven transient transfection techniques for primary strains and continuous lines of keratinocytes. The emphasis is on readily available and inexpensive resources that also allow for repeatability and adaptability to scale, experimental conditions, and sample ...
Anthony M, Flores, Brian J, Aneskievich
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Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carriers.
Advanced Drug Delivery Reviews, 2008Igor I. Slowing +3 more
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Transfection of Lymphoid Cells
Current Protocols in Immunology, 1994AbstractUsing available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more ...
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Dose‐Independent Transfection of Hydrophobized Polyplexes
Advanced Materials, 2021Nasha Qiu, Zhuxian Zhou, Jianbin Tang
exaly
Nihon rinsho. Japanese journal of clinical medicine, 1997
Transfection is a method to introduce DNA into mammalian cells. Calcium phosphate-mediated transfection is well used as one of the methods for transfection. The basic protocol is simple and does not require the elaborated instruments. It has been applied to introduce the heterogeneous DNA for integration into and expression from the chromosome.
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Transfection is a method to introduce DNA into mammalian cells. Calcium phosphate-mediated transfection is well used as one of the methods for transfection. The basic protocol is simple and does not require the elaborated instruments. It has been applied to introduce the heterogeneous DNA for integration into and expression from the chromosome.
openaire +1 more source