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LncRNA TUG1 promotes hypertrophic scar formation via the miR-627/IGF1R axis. [PDF]
J Mol HistolBai Z +9 more
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Workflows for stable cell line generation: Chemical transfection and viral transduction. [PDF]
Mol CellsMin C, Kim J, Lee G.
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Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis
Journal of Gene Medicine, 2005Mini Thomas, Robert Langer
exaly +2 more sources
Materials for Improving Immune Cell Transfection
Advances in Materials, 2021Chimeric antigen receptor T cell (CAR‐T) therapy holds great promise for preventing and treating deadly diseases such as cancer. However, it remains challenging to transfect and engineer primary immune cells for clinical cell manufacturing.
Arun R K Kumar +4 more
semanticscholar +1 more source
Malaria transfection and transfection vectors
Trends in Parasitology, 2003Malaria remains a leading cause of death due to infectious disease. The completion of the Plasmodium falciparum genome sequencing project and release of preliminary proteomics data have significantly increased our understanding of the biology of this organism.
Gardiner, Donald L. +3 more
openaire +5 more sources
Transfection by Electroporation
Current Protocols in Molecular Biology, 2010AbstractElectroporation—the use of high‐voltage electric shocks to introduce DNA into cells—can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques.
Huntington, Potter, Richard, Heller
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Cold Spring Harbor Protocols, 2018
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
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Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar +2 more
openaire +2 more sources
Nature Medicine, 1995
The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
D M, Wesson, D J, Krogstad
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The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
D M, Wesson, D J, Krogstad
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2018
Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
openaire +2 more sources
2003
Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
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Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
openaire +2 more sources