Results 251 to 260 of about 733,470 (308)
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Current Protocols in Neuroscience, 1997
AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
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AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
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2019
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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2011
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
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Transfection of Difficult-to-Transfect Primary Mammalian Cells
2011Primary cells are a valuable tool for researchers and are often preferred over transformed or immortalized cell lines since they are biologically more relevant and resemble the in vivo situation much closer. Unfortunately, efficient gene transfer in primary cells is still limited.
Oliver, Gresch, Ludger, Altrogge
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Journal of Cellular Biochemistry, 2018
AbstractCell‐penetrating peptides (CPPs) are used to internalize different cargoes, including DNA, into live mammalian and plant cells. Despite many cells being easily transfected with this approach, other cells are rather “difficult” or “hard to transfect,” including protist cells of the genus Leishmania.
Andrea‐Anneliese Keller +6 more
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AbstractCell‐penetrating peptides (CPPs) are used to internalize different cargoes, including DNA, into live mammalian and plant cells. Despite many cells being easily transfected with this approach, other cells are rather “difficult” or “hard to transfect,” including protist cells of the genus Leishmania.
Andrea‐Anneliese Keller +6 more
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2003
Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Cationic Lipids for Transfection
Current Medicinal Chemistry, 2003Among other strategies, the use of cationic lipids as autoassembling vehicles for non viral DNA transfection has received considerable attention. An exponentially growing litterature has been published on this topic (over 700 hits for the past decade, including 400 in the last two years). The present review focuses on the main present strategies aiming
Céline, Nicolazzi +4 more
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Transfection with baculovirus DNA
Virology, 1980Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973).
J P, Burand, M D, Summers, G E, Smith
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Current Medicinal Chemistry, 2003
This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Dexi, Liu, Tan, Ren, Xiang, Gao
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This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Dexi, Liu, Tan, Ren, Xiang, Gao
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Microbial Transformation and Transfection
Annual Review of Microbiology, 1966FORMATION OF COMPETENCE STATE 375 Physiological state of culture.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 375 Evidence for wall changes accompanying competence . . . . . . . . . . . . . . . . . . . .. 377 EVIDENCE FOR A DNA RECEPTOR OR COMPETENCE "FACTOR". 378 CHARACTERISTICS OF BIOLOGICALLY ACTIVE DNA.
J, Spizizen, B E, Reilly, A H, Evans
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