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Liposome-Mediated Transfection

Cold Spring Harbor Protocols, 2011
INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology. Cationic liposomes are the most widely used class of carrier for transfection and a number of products are available commercially: Lipofectin (a 1:1 mixture of DOTMA [N-[1(2,3-dioleoyloxy)propyl]-N,N,N ...
David A, Dean, Joshua Z, Gasiorowski
openaire   +2 more sources

Liposome‐Mediated Transfection

Current Protocols in Immunology, 1992
AbstractUsing liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the describes a transient expression system while an involves stable transformation and expression of DNA integrated into the genome of the transfected cell.
Michael, Whitt   +2 more
openaire   +2 more sources

Transfection of Lymphoid Cells

Current Protocols in Immunology, 1994
AbstractUsing available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more ...
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Protamine-Based Strategies for RNA Transfection

Pharmaceutics, 2021
Mark Mellett, Steve Pascolo
exaly  

[Transfection].

Nihon rinsho. Japanese journal of clinical medicine, 1997
Transfection is a method to introduce DNA into mammalian cells. Calcium phosphate-mediated transfection is well used as one of the methods for transfection. The basic protocol is simple and does not require the elaborated instruments. It has been applied to introduce the heterogeneous DNA for integration into and expression from the chromosome.
openaire   +1 more source

Gemini Surfactants: New Synthetic Vectors for Gene Transfection

Angewandte Chemie - International Edition, 2003
Roeland Nolte   +2 more
exaly  

Plasmid Transfection in Mammalian Cells Spatiotemporally Tracked by a Gold Nanoparticle

ACS Nano, 2015
Kate J F Carnevale   +2 more
exaly  

Mechanism of cell transfection with plasmid/chitosan complexes

Biochimica Et Biophysica Acta - Biomembranes, 2001
Tsuyoshi Ishii, Toshinori Sato
exaly  

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