Results 341 to 350 of about 968,391 (388)
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2018
Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
openaire +2 more sources
2019
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
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Liposome‐Mediated Transfection
Current Protocols in Immunology, 1992AbstractUsing liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the describes a transient expression system while an involves stable transformation and expression of DNA integrated into the genome of the transfected cell.
Michael, Whitt +2 more
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Nature Medicine, 1995
The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
D M, Wesson, D J, Krogstad
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The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
D M, Wesson, D J, Krogstad
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Liposome-Mediated Transfection
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology. Cationic liposomes are the most widely used class of carrier for transfection and a number of products are available commercially: Lipofectin (a 1:1 mixture of DOTMA [N-[1(2,3-dioleoyloxy)propyl]-N,N,N ...
David A, Dean, Joshua Z, Gasiorowski
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Current Medicinal Chemistry, 2003
This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Dexi, Liu, Tan, Ren, Xiang, Gao
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This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Dexi, Liu, Tan, Ren, Xiang, Gao
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Calcium Phosphate Transfection
Current Protocols in Neuroscience, 1997AbstractThis unit presents two methods of calcium phosphate‐based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface.
R E, Kingston, C A, Chen, H, Okayama
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2003
Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Cold Spring Harbor Protocols, 2018
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Priti, Kumar +2 more
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Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Priti, Kumar +2 more
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Dendrimer-Mediated Transfection
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology. Polymer-based systems can be used to transfect cells, some of which rely on one type of molecule, whereas others contain a mixture of polymers and lipids. Reagents such as SuperFect or PolyFect use activated
David A, Dean, Joshua Z, Gasiorowski
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