Results 361 to 370 of about 928,471 (414)

Targeting the dual miRNA/BMP2 network: LncRNA H19-mediated temozolomide resistance unveils novel therapeutic strategies in glioblastoma. [PDF]

Front Oncol
Chen Q, Zhuang S, Chen S, Wu B, Zhou Q, Wang W.   +5 more
europepmc   +1 more source

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Materials for Improving Immune Cell Transfection

Advances in Materials, 2021
Chimeric antigen receptor T cell (CAR‐T) therapy holds great promise for preventing and treating deadly diseases such as cancer. However, it remains challenging to transfect and engineer primary immune cells for clinical cell manufacturing.
Arun R K Kumar, Yufeng Shou, Brian Chan, Krishaa L, A. Tay   +4 more
semanticscholar   +1 more source

Malaria transfection and transfection vectors

Trends in Parasitology, 2003
Malaria remains a leading cause of death due to infectious disease. The completion of the Plasmodium falciparum genome sequencing project and release of preliminary proteomics data have significantly increased our understanding of the biology of this organism.
Gardiner, Donald L., Skinner-Adams, Tina S., Spielmann, Tobias, Trenholme, Katharine R.   +3 more
openaire   +4 more sources

Transfection and malaria

Nature Medicine, 1995
The introduction of novel genes into mosquitoes and malaria parasites is now feasible, raising hopes for an effective attack on malaria.
Donald J. Krogstad, Dawn M. Wesson
openaire   +3 more sources

Calcium Phosphate Transfection

Current Protocols in Neuroscience, 1997
AbstractThis unit presents two methods of calcium phosphate‐based eukaryotic cell transfection that can be used for both transient and stable transfections. In these protocols, plasmid DNA is introduced to monolayer cell cultures via a precipitate that adheres to the cell surface.
John K. Rose, Claudia A. Chen, Claudia A. Chen, Robert E. Kingston   +3 more
openaire   +8 more sources

Optical Transfection

Cold Spring Harbor Protocols, 2018
Strategies for the delivery of genes into eukaryotic cells fall into three categories: transfection by biochemical methods, transfection by physical methods, and virus-mediated transduction. “Optical transfection”—a physical transfection method—exploits the ability of light to create small transient pores in the plasma membrane of mammalian cells.
Priti, Kumar, Arvindhan, Nagarajan, Pradeep D, Uchil   +2 more
openaire   +2 more sources

Transduction and transfection of difficult‐to‐transfect cells: Systematic attempts for the transfection of protozoa Leishmania [PDF]

Journal of Cellular Biochemistry, 2018
AbstractCell‐penetrating peptides (CPPs) are used to internalize different cargoes, including DNA, into live mammalian and plant cells. Despite many cells being easily transfected with this approach, other cells are rather “difficult” or “hard to transfect,” including protist cells of the genus Leishmania.
Andrea‐Anneliese Keller, Berith Scheiding, Reinhard Breitling, Andreas Licht, Peter Hemmerich, Stefan Lorkowski, Siegmund Reissmann   +6 more
openaire   +2 more sources

Optimization of Transfection

Current Protocols in Neuroscience, 1997
AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection.
openaire   +4 more sources

High DNA-Binding Affinity and Gene-Transfection Efficacy of Bioreducible Cationic Nanomicelles with a Fluorinated Core.

Angewandte Chemie, 2016
During the last two decades, cationic polymers have become one of the most promising synthetic vectors for gene transfection. However, the weak interactions formed between DNA and cationic polymers result in low transfection efficacy.
Long-Hai Wang, De-Cheng Wu, Hang Xu, Yezi You   +3 more
semanticscholar   +1 more source