Results 371 to 380 of about 928,471 (414)
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2003
Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
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Recent improvements in the biolistic technique and devices have increased its usefulness for transfection of neurons. With these recent advances, both dissociated and slice cultures can be transfected at reasonably high rates. This chapter focuses on the parameters that determine the successful biolistic transfection of neurons in both types of ...
Paul C, Bridgman +2 more
openaire +2 more sources
2018
Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
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Transfection is defined as the transfer of foreign nucleic acids into cells. In general, transfection may achieve either overexpression of a gene by the transfer of plasmid DNA or suppression of gene expression by RNA interference after transfer of small interfering RNA.
Andrea-Anneliese, Keller +4 more
openaire +2 more sources
2011
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
openaire +2 more sources
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. As a nonviral delivery system, transfection has gained popularity. The cost-effective and rapid production of recombinant human therapeutic proteins in sufficient quantities has been one of the challenges faced in biopharmaceutical ...
Chahal, P., Durocher, Y., Kamen, A.
openaire +2 more sources
Transfection of Difficult-to-Transfect Primary Mammalian Cells
2011Primary cells are a valuable tool for researchers and are often preferred over transformed or immortalized cell lines since they are biologically more relevant and resemble the in vivo situation much closer. Unfortunately, efficient gene transfer in primary cells is still limited.
Oliver Gresch, Ludger Altrogge
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Liposome‐Mediated Transfection
Current Protocols in Immunology, 1992AbstractUsing liposomes to deliver DNA into different eukaryotic cell types results in higher efficiency and greater reproducibility than other transfection methods. In this unit, the describes a transient expression system while an involves stable transformation and expression of DNA integrated into the genome of the transfected cell.
Linda Buonocore +2 more
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PepFect14 Signaling and Transfection
2021PepFect14 is a cell-penetrating peptide (CPP) derived from stearylated transportan-10 (strearil-TP10) with which it shares the stearic acid residue on C' terminus and the amino acid sequence except for lysines that in PepFect14 are substituted with ornithines.
Luca Falato +3 more
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Biomacromolecules, 2016
A successful polymeric gene delivery vector is denoted by both transfection efficiency and biocompatibility. However, the existing vectors with combined high efficacy and minimal toxicity still fall short.
Yongsheng Gao +6 more
semanticscholar +1 more source
A successful polymeric gene delivery vector is denoted by both transfection efficiency and biocompatibility. However, the existing vectors with combined high efficacy and minimal toxicity still fall short.
Yongsheng Gao +6 more
semanticscholar +1 more source
2019
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
openaire +2 more sources
Transfection is a process of introducing nucleic acid into eukaryotic cells using various chemical or physical methods. Transduction is another popular transfection technology that uses viral vectors to deliver foreign genes. In the past decade, transfection technologies have evolved tremendously, for stable clone generation, therapeutic protein ...
Do Minh, A. +3 more
openaire +2 more sources
Liposome-Mediated Transfection
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe efficient delivery of exogenous DNA to cells for expression and function studies is an essential technique of modern cell biology. Cationic liposomes are the most widely used class of carrier for transfection and a number of products are available commercially: Lipofectin (a 1:1 mixture of DOTMA [N-[1(2,3-dioleoyloxy)propyl]-N,N,N ...
Joshua Z. Gasiorowski, David A. Dean
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2017
Over the last 30 years, the transfection of DNA or RNA into mammalian cells has evolved into an ever more sophisticated research tool for the investigation of gene expression and protein function. Transfection is used to describe the process of introducing foreign nucleic acid into a cell.
Semira Sheikh +2 more
openaire +2 more sources
Over the last 30 years, the transfection of DNA or RNA into mammalian cells has evolved into an ever more sophisticated research tool for the investigation of gene expression and protein function. Transfection is used to describe the process of introducing foreign nucleic acid into a cell.
Semira Sheikh +2 more
openaire +2 more sources