Results 381 to 390 of about 928,471 (414)
Some of the next articles are maybe not open access.
Current Medicinal Chemistry, 2003
This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Tan Ren, Xiang Gao, Dexi Liu
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This review focuses on the recent developments in study of cationic lipids as carriers for DNA delivery. Emphasis is placed on a class of compounds as exemplifies by their similarity in structures and transfection activities. The technical aspects are also reviewed on how to prepare DNA-lipid complexes and to perform transfection. A brief discussion of
Tan Ren, Xiang Gao, Dexi Liu
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Lab on a Chip, 2015
Current transfection technologies lead to significant inter-clonal variations. Previously we introduced a unique electrotransfection technology, Nanochannel-Electroporation (NEP), which can precisely and benignly transfect small cell populations (~100 ...
Lingqian Chang +12 more
semanticscholar +1 more source
Current transfection technologies lead to significant inter-clonal variations. Previously we introduced a unique electrotransfection technology, Nanochannel-Electroporation (NEP), which can precisely and benignly transfect small cell populations (~100 ...
Lingqian Chang +12 more
semanticscholar +1 more source
Transfection with baculovirus DNA
Virology, 1980Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973).
Max D. Summers +2 more
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The influence of polymer architecture on in vitro pDNA transfection.
Journal of materials chemistry. B, 2015In 2012, the first gene therapy agent was approved by the Europe Medicines Agency leading to increased interest in this research field. Beside viruses, non-viral agents based on lipids or polymers represent aspiring alternatives to deliver the genetic ...
Alexandra C. Rinkenauer +3 more
semanticscholar +1 more source
Biolistic Transfection of Neurons
Science's STKE, 2000One method used to study gene function is through the manipulation of gene expression by transfecting cells with DNA constructs designed to overexpress or knock out particular proteins. Unfortunately, transfection of cells and tissues remains a rate-limiting step for molecular studies in many fields, especially neurobiology.
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Dendrimers in gene transfection
Biochemistry (Moscow), 2009Dendrimers are a new class of nanocomposite materials. They are branching polymers whose structure is formed by monomeric subunit branches diverging to all sides from a central nucleus. The type of nucleus, attached monomers, and functional groups can be chosen during synthesis, which produces dendrimers of definite size, shape, density, polarity ...
Maria Bryszewska +2 more
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Transfection of Malaria Parasites
Methods, 1997The stable genetic transformation of three phylogenetically diverse species of Plasmodium, the parasitic etiological agent of malaria, is now possible. The parasite is haploid throughout the vast majority of its life cycle. Therefore with the single selectable marker activity and protocols currently available, it is possible not only to express ...
Andrew P. Waters +3 more
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DNA Transfection by Electroporation
Cold Spring Harbor Protocols, 2019Electroporation, which uses pulsed electrical fields, can be used to introduce DNA into a variety of animal cells, plant cells, and bacteria. Electroporation works well with cell lines that are refractory to other transfection techniques, such as lipofection and calcium phosphate–DNA coprecipitation. But, as with other transfection methods, the optimal
Arvindhan Nagarajan +2 more
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Cold Spring Harbor Protocols, 2018
Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Pradeep D. Uchil +2 more
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Biochemical methods of transfection, including calcium phosphate-mediated and diethylaminoethyl (DEAE)-dextran-mediated transfection, have been used for many years to deliver nucleic acids into cultured eukaryotic cells. Here, we briefly review the use of DEAE-dextran in transfection.
Pradeep D. Uchil +2 more
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Microbial Transformation and Transfection
Annual Review of Microbiology, 1966FORMATION OF COMPETENCE STATE 375 Physiological state of culture.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 375 Evidence for wall changes accompanying competence . . . . . . . . . . . . . . . . . . . .. 377 EVIDENCE FOR A DNA RECEPTOR OR COMPETENCE "FACTOR". 378 CHARACTERISTICS OF BIOLOGICALLY ACTIVE DNA.
A H Evans, J Spizizen, B E Reilly
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