Results 391 to 400 of about 928,471 (414)
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Polyamidoamine cascade polymers mediate efficient transfection of cells in culture.
Bioconjugate chemistry, 1993Cascade polymers also known as Starburst dendrimers are spheroidal polycations that can be synthesized with a well-defined diameter and a precise number of terminal amines per dendrimer.
J. Haensler, F. Szoka
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Transfection of Lymphoid Cells
Current Protocols in Immunology, 1994AbstractUsing available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more ...
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Mechanism of DNA release from cationic liposome/DNA complexes used in cell transfection.
Biochemistry, 1996To understand how DNA is released from cationic liposome/DNA complexes in cells, we investigated which biomolecules mediate release of DNA from a complex with cationic liposomes.
Yuhong Xu, F. Szoka
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2003
Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Prior to the introduction of transgene constructs into animals, it is useful first to determine the presence of a functional promoter and transcription unit. This can be achieved by transient transfection of the construct into heterologous cell lines and subsequent measurement of reporter enzyme activity in cell extracts.
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Nanostraw-electroporation system for highly efficient intracellular delivery and transfection.
ACS Nano, 2013Nondestructive introduction of genes, proteins, and small molecules into mammalian cells with high efficiency is a challenging, yet critical, process.
Xi Xie +5 more
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Transfection of Plasmodium falciparum
2012Genetic manipulation of Plasmodium falciparum remains very challenging, mainly due to the parasite genome's high A/T-richness and low transfection efficiency. This chapter includes methods for generating transient and stable transfections by electroporation, allelic replacement with tagged genes, gene deletion, and the analysis of all the above.
Melanie Rug, Alexander G. Maier
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Graphene based gene transfection.
Nanoscale, 2011Graphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we successfully use graphene as a non-toxic nano-vehicle for efficient gene
Liangzhu Feng, Shuai Zhang, Zhuang Liu
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Cationic Lipids for Transfection
Current Medicinal Chemistry, 2003Among other strategies, the use of cationic lipids as autoassembling vehicles for non viral DNA transfection has received considerable attention. An exponentially growing litterature has been published on this topic (over 700 hits for the past decade, including 400 in the last two years). The present review focuses on the main present strategies aiming
Michel Bessodes +4 more
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Virology, 1977
Abstract The frequency of transfection with Mu-DNA in a RecBC, SbcB strain treated with CaCl 2 is extremely low. The frequency of transfection is 100- to 1000-fold higher when, prior to the CaCl 2 treatment, early Mu functions are expressed in the recipient cells. It was found that the products of the early Mu genes A and B , which are necessary
Gerth C. Westmaas +2 more
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Abstract The frequency of transfection with Mu-DNA in a RecBC, SbcB strain treated with CaCl 2 is extremely low. The frequency of transfection is 100- to 1000-fold higher when, prior to the CaCl 2 treatment, early Mu functions are expressed in the recipient cells. It was found that the products of the early Mu genes A and B , which are necessary
Gerth C. Westmaas +2 more
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Transient transfection of DT40
2006Transient transfection of cell lines can be extremely useful in a number of applications. However, until recently, obtaining useful and reproducible percentages of transiently expressing DT40 was almost impossible. Standard techniques such as electroporation usually give low transfection efficiencies (can be up to 40%, but ususally less than 5%) while ...
Julian E. Sale, Roger Franklin
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