Results 261 to 270 of about 280,113 (289)

Improved transfection efficiency of CS/DNA complex by co-transfected chitosanase gene

International Journal of Pharmaceutics, 2008
Previously, we had demonstrated that insufficient intracellular unpacking of exogene from its chitosan carrier contributes towards the restricted transfection efficiency of CS/DNA complex. In order to enhance intracellular unpacking and thus improve the transfection efficiency, our present work has addressed a novel strategy of chitosanase gene (csn ...
Aijun, Zuo, Pei, Sun, Dongchun, Liang, Wenguang, Liu, Ronglan, Zhao, Gang, Guo, Nan, Cheng, Jingyu, Zhang, Kangde, Yao   +8 more
openaire   +2 more sources

Efficient transfection of chicken cells by lipofection, and introduction of transfected blastodermal cells into the embryo

Molecular Reproduction and Development, 1991
AbstractChicken blastodermal cells (CBCs) and primary chicken fibroblasts (PCFs) have been lipofected with a variety of lacZ constructs encoding Escherichia coli β‐galactosidase (β‐gal). A reporter construct (phspPTlacZpA) containing a mouse heat‐shock protein 68 gene (hsp 68) promoter was used to establish conditions for efficient lipofection.
C L, Brazolot, J N, Petitte, R J, Etches, A M, Verrinder Gibbins   +3 more
openaire   +2 more sources

Transfection of embryonal carcinoma cells at high efficiency using liposome‐mediated transfection

Molecular Reproduction and Development, 2002
AbstractEmbryonal carcinoma (EC) cells are recognized as an excellent model system for studying the early stages of mammalian development. Many studies performed with EC cells involve transient transfection with promoter/reporter gene constructs and/or mammalian expression vectors.
Tamara, Nowling, Michelle, Desler, Charles, Kuszynski, Angie, Rizzino   +3 more
openaire   +2 more sources

Synthesis and Transfection Efficiencies of New Lipophilic Polyamines

Journal of Medicinal Chemistry, 2006
A homologous series of lipophilic polyamines was synthesized and evaluated for DNA delivery and transfection efficiency. The series contained 1,4-butanediamine, 1,8-octanediamine, 2-[2-(2-amino-ethoxy)-ethoxy]-ethylamine, homospermidine, and homospermine covalently attached via their N1 terminus to a 3,4-bis(oleyloxy)-benzyl motif.
Gardner, Richard Andrew, Belting, Mattias, Svensson, Katrin, Phanstiel IV, Otto   +3 more
openaire   +3 more sources

High efficiency transfection of Plasmodium berghei facilitates novel selection procedures [PDF]

Molecular and Biochemical Parasitology, 2006
Contains fulltext : 49370.pdf (Publisher’s version ) (Closed access)The use of transfection in the study of the biology of malaria parasites has been limited due to poor transfection efficiencies (frequency of 10(-6) to 10(-9)) and a ...
Blandine Franke-Fayard, Kai Matuschewski, Andy Waters   +2 more
exaly   +2 more sources

Factors that affect the efficiency of cell transfection by immunoporation

Analytical Biochemistry, 2004
Immunoporation is a recently discovered method that is able to transfect various human cell lines efficiently by targeting the cell surface antigens with antibody-coated beads. For this particular study, HL60, a cell line difficult to transfect by other methods, was used as a model to define the various parameters of the cell membrane that determine ...
C, Tzavelas, L, Bildirici, D, Rickwood
openaire   +2 more sources

High-Efficiency Nonviral Transfection of Primary Chondrocytes

2004
The introduction of foreign DNA into mammalian cells is an essential investigative tool in molecular biology. Nonviral approaches to transfection offer the advantage of relatively simple vector design, production, and purification and, for tissue engineering applications, avoid many of the potential risks associated with virus-mediated transfection ...
Jean F, Welter, Luis A, Solchaga, Matthew C, Stewart   +2 more
openaire   +2 more sources

High-efficiency gene transfection of macrophages by lipoplexes

International Journal of Pharmaceutics, 2000
Macrophage transfection studies are crucial for understanding gene regulation and expression. However, gene transfection in macrophages is difficult. We have shown here that macrophages are more resistant to gene transfection compared with other cell types.
S, Dokka, D, Toledo, X, Shi, J, Ye, Y, Rojanasakul   +4 more
openaire   +2 more sources

Improving the transfection efficiency of post-mitotic neurons

Journal of Neuroscience Methods, 2001
The transfection of post-mitotic cells, including primary cortical and hippocampal neurons, has proven for the most part to be inefficient. Methods such as DNA/Ca++ phosphate co-precipitation, electroporation, cationic lipids and micro-injection are often toxic to the cell and rarely give a transfection efficiency exceeding 3% of the surviving culture.
E C, Ohki, M L, Tilkins, V C, Ciccarone, P J, Price   +3 more
openaire   +2 more sources

Non-viral Methodology for Efficient Co-transfection

2018
The potential impact of CRISPR/Cas9, TALE, and zinc finger technology is immense, both with respect to their use as tools for understanding the roles and functions of the genomic elements and epigenome modifications in an endogenous context and as new methods for treatment of diseases.
Jessica A, Kretzmann, Cameron W, Evans, Marck, Norret, Pilar, Blancafort, K, Swaminathan Iyer   +4 more
openaire   +2 more sources