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Improved transfection efficiency of chitosan‐DNA complexes employing reverse transfection
Journal of Applied Polymer Science, 2011AbstractThe present study was designed to systematically compare the conventional and reverse transfection methodologies for chitosan/DNA complexes using a low molecular weight (MW) chitosan. The hydrodynamic diameter of the complexes, measured by Dynamic Light Scattering (DLS) was found to be ∼ 216 nm and TEM investigations showed spherical and ...
Surendra Nimesh +3 more
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The gene transfection efficiency of a folate–PEI600–cyclodextrin nanopolymer
Biomaterials, 2009The success of gene therapy relies on a safe and effective gene delivery system. In this communication, we describe the use of folate grafted PEI(600)-CyD (H(1)) as an effective polyplex-forming plasmid delivery agent with low toxicity. The structures of the polymer and polyplex were characterized, and the in vitro transfection efficiency, cytotoxicity,
Chow, BKC +9 more
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Efficient Cell Transfection with Melamine-Based Gemini Surfactants
Bioconjugate Chemistry, 2013Gemini surfactants consisting of two melamine scaffolds connected by a n-hexyl linker and functionalized with a 1-propylammonium polar head and a lipophilic chain having variable carbon length (from C8 to C16) were synthesized. These were then used successfully for the transfection of A549, U87 MG, and Bristol 8 cell lines with maxGFP expressing ...
Perrone S. +5 more
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Enhancement of Transfection Efficiency by Using Oligodeoxyribonucleotide as Carrier
Analytical Biochemistry, 1997M C, Lai, Y C, Wang, F Y, Yang, L C, Au
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Lipoplex morphologies and their influences on transfection efficiency in gene delivery
Journal of Controlled Release, 2007Shubiao Zhang
exaly