Results 21 to 30 of about 3,618,105 (334)

Altering under-represented DNA sequences elevates bacterial transformation efficiency

mBio, 2023
A cornerstone of bacterial molecular biology is the ability to genetically manipulate the microbe under study. Many bacteria are difficult to manipulate genetically, a phenotype due in part to robust removal of newly acquired DNA, for example, by ...
Shuai Hu, Stefani Giacopazzi, Ryan Modlin, Kevin Karplus, David L. Bernick, Karen M. Ottemann   +5 more
doaj   +1 more source

Disclosing the ecological implications of heavy metal disturbance on the microbial N-transformation process in the ocean tidal flushing urban estuary

Ecological Indicators, 2022
Anthropogenic source of heavy metals in the estuary-coastal sediment could cause serious environmental concerns. Thus, much needs to be concerned about the linkages between bacterial community, microbial nitrogen processes and heavy metals accumulation ...
Caixia Wang, Jiujun Ju, Haikun Zhang, Pengyuan Liu, Qiusheng Zheng, Xiaoke Hu   +5 more
doaj   +1 more source

Multisite transformation in Neisseria gonorrhoeae: insights on transformations mechanisms and new genetic modification protocols

Frontiers in Microbiology, 2023
Natural transformation, or the uptake of naked DNA from the external milieu by bacteria, holds a unique place in the history of biology. This is both the beginning of the realization of the correct chemical nature of genes and the first technical step to
Vui Yin Seow, Vui Yin Seow, Vui Yin Seow, Olga Tsygelnytska, Nicolas Biais, Nicolas Biais, Nicolas Biais   +6 more
doaj   +1 more source

Horizontal DNA transfer mechanisms of bacteria as weapons of intragenomic conflict [PDF]

, 2016
Horizontal DNA transfer (HDT) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial.
A Adams, A Adams, AB Dalia, AI Khan, B Martin, BJ Haijema, BR Levin, C Boutry, C Buchrieser, C Camacho, C Canchaya, C Chewapreecha, C Fraser, C Johnston, C Johnston, C Johnston, Christophe Fraser, Christopher Wymant, CS Wylie, D Dubnau, D Hyatt, DA Baltrus, DA Caugant, DA Higgins, DA Morrison, DA Morrison, DE Fouts, DJP Engelmoer, E Borezee, E Szathmáry, E V Pestova, E Zaccaria, EA Campbell, EJ Feil, EJ Gaasbeek, EJ Gaasbeek, EP Rocha, ES Antonova, F Ginetti, F Griffith, F Liu, GG Wilson, GJ Stewart, GM Süel, GW Goldberg, H Bagci, H Bernstein, H Deveau, H Li, H Maamar, H Muller, H Ochman, H Tong, H-H Chou, HC Neu, I Chen, I Mortier-Barriere, I Mortier-Barrière, J Bondy-Denomy, J Guglielmini, J Majewski, J Majewski, J-P Claverys, JA Lanie, JA Londoño-Vallejo, JA Mongold, JC Lefevre, JC Mell, JH Stuy, JJ Sakamoto, JK Setlow, JM Auchtung, JM Musser, JM Smith, JM Smith, JO McInerney, JP Claverys, JP Claverys, JT Simpson, JW Beaber, K Turgay, KS Makarova, KT Konstantinidis, L Attaiech, L Fontaine, L Van Valen, L-M Bobay, LP Macfadyen, LS Frost, LS Håvarstein, LS Håvarstein, M Berge, M Boetzer, M Del Grosso, M Prudhomme, M Ramirez, MA Hoelzer, ME Kahn, MF Wojciechowski, MJ Lopez-Sanchez, ML Gwinn, MR Oggioni, MS Dorer, MTG Holden, Nicholas J. Croucher, Nick H. Barton, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, O Johnsborg, OE Cornejo, OH Ambur, OT Avery, P Glaser, P Jorth, P Luo, PA Levin, Paul Turner, PJ Johnsen, R Davidoff-Abelson, R Edgar, R Mostowy, RA Fisher, Rafal Mostowy, RE Michod, RE Yasbin, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RK Aziz, RP Novick, S Lacks, SA McMahon, SB Beres, SE Finkel, SJ Labrie, SP Otto, Stephen D. Bentley, T Carver, T Nozawa, U Munoz-Najar, WA Sweetman, WJ Kent, X Charpentier, X Charpentier, X Didelot, Y Li, Z Zhou   +157 more
core   +3 more sources

Seasonal changes in microbial dissolved organic sulfur transformations in coastal waters [PDF]

, 2020
The marine trace gas dimethylsulfide (DMS) is the single most important biogenic source of atmospheric sulfur, accounting for up to 80% of global biogenic sulfur emissions.
Dixon, Joanna L., Hopkins, Frances E., Schäfer, Hendrik, Stephens, John A.   +3 more
core   +1 more source

Helix Matrix Transformation Combined With Convolutional Neural Network Algorithm for Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Bacterial Identification

Frontiers in Microbiology, 2020
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis is a rapid and reliable method for bacterial identification.
Jin Ling, Jin Ling, Gaomin Li, Gaomin Li, Hong Shao, Hong Shao, Hong Wang, Hong Wang, Hongrui Yin, Hongrui Yin, Hu Zhou, Yufei Song, Gang Chen, Gang Chen   +13 more
doaj   +1 more source

Draft genome sequence of the naturally competent Bacillus simplex strain WY10 [PDF]

, 2017
We sequenced a naturally competent bacterial isolate, WY10, cultured from a Wyoming soil sample. Sequence analysis revealed that WY10 is a novel strain of Bacillus simplex .
Adhya, Sankar L, Bliskovsky, Valery V, Dantas, Gautam, Keen, Eric C   +3 more
core   +2 more sources

Bacterial Diversity Controls Transformation of Wastewater-Derived Organic Contaminants in River-Simulating Flumes

Environmental Science and Technology, 2020
Hyporheic zones are the water-saturated flow-through subsurfaces of rivers which are characterized by the simultaneous occurrence of multiple physical, biological, and chemical processes. Two factors playing a role in the hyporheic attenuation of organic
M. Posselt, Jonas Mechelke, Cyrus Rutere, C. Coll, A. Jaeger, M. Raza, Karin Meinikmann, S. Krause, Anna Sobek, J. Lewandowski, M. Horn, J. Hollender, Jonathan P. Benskin   +12 more
semanticscholar   +1 more source

Simulating microbial degradation of organic matter in a simple porous system using the 3-D diffusion-based model MOSAIC [PDF]

, 2013
This paper deals with the simulation of microbial degradation of organic matter in soil within the pore space at a microscopic scale. Pore space was analysed with micro-computed tomography and described using a sphere network coming from a geometrical ...
Chenu, Claire, Coucheney, E., Garnier, Patricia, Monga, O., Nunan, N., Otten, Wilfred, Pot, V.   +6 more
core   +7 more sources

Bacterial transformation v1 [PDF]

, 2019
Transformation of competent Escherichia coli cultures with heterologous DNA. This protocol allows the introduction of external DNA into competent E. coli cells. Typically, the piece of DNA consists of a plasmid, either for its cloning or for heterologous protein expression.
Dana Mozaffari, Laura Kvedarauskaite
  +6 more sources