Results 71 to 80 of about 3,618,105 (334)

OS EXPERIMENTOS DE GRIFFITH NO ENSINO DE BIOLOGIA: A TRANSPOSIÇÃO DIDÁTICA DO CONCEITO DE TRANSFORMAÇÃO NOS LIVROS DIDÁTICOS

open access: yesEnsaio Pesquisa em Educação em Ciências, 2010
Griffith's experiments regarding bacterial transformation Pneumococcus are regarded as essential when related to DNA/heredity recognition process. The current study goal is to assess the didactic transposition of the concept of bacterial transformation ...
Caroline Belotto Batisteti   +2 more
doaj  

Interspecies DNA acquisition by a naturally competent Acinetobacter baumannii strain [PDF]

open access: yes, 2019
The human pathogen Acinetobacter baumannii possesses high genetic plasticity and frequently acquires antimicrobial resistance genes. Here we investigated the role of natural transformation in these processes. Genomic DNA from different sources, including
Bahiense, Camila dos Santos   +12 more
core   +1 more source

Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency

open access: yesScientific Reports, 2016
Bacterial competent cells are essential for cloning, construction of DNA libraries, and mutagenesis in every molecular biology laboratory. Among various transformation methods, electroporation is found to own the best transformation efficiency.
Qiang Tu   +13 more
semanticscholar   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

Soft agar colony formation assay to quantify mouse embryonic fibroblast transformation after Salmonella infection

open access: yesSTAR Protocols, 2023
Summary: Links between bacterial infections and cancer are actively investigated. Cost-effective assays to quantify bacterial oncogenic potential can shed new light on these links.
Virginie Stévenin, Jacques Neefjes
doaj   +1 more source

T-DNA promoter tagging in Nicotiana tabacum : a thesis presented in fulfilment of the requirements for the degree of Master of Philosophy in Genetics at Massey University, Palmerston North, New Zealand [PDF]

open access: yes, 1997
Plant development is primarily controlled at the level of gene expression. In order to analyse this regulation it is necessary to isolate genes which are involved in organ development through cellular and tissue determination or which respond to ...
Kerr, Donald Haldane
core  

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

open access: yesFEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo   +2 more
wiley   +1 more source

Transfer of antibiotic resistance in Staphylococcus aureus [PDF]

open access: yes, 2017
Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera.
Haaber, Jakob   +2 more
core   +1 more source

Genome sequence and plasmid transformation of the model high-yield bacterial cellulose producer Gluconacetobacter hansenii ATCC 53582

open access: yesScientific Reports, 2016
Bacterial cellulose is a strong, highly pure form of cellulose that is used in a range of applications in industry, consumer goods and medicine. Gluconacetobacter hansenii ATCC 53582 is one of the highest reported bacterial cellulose producing strains ...
Micah J Florea   +4 more
semanticscholar   +1 more source

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