Results 61 to 70 of about 1,026,560 (278)

Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates. [PDF]

, 2019
Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples ...
Carlson, Hans K, Deutschbauer, Adam M, Kothari, Ankita, Mukhopadhyay, Aindrila, Soneja, Drishti, Tang, Albert   +5 more
core   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Establishment of A Simple and Efficient Agrobacterium-mediated Genetic Transformation System to Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Horticultural Plant Journal, 2021
Chinese cabbage, belonging to Brassica rapa species, is an important vegetable in Eastern Asia. It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low.
Xiaonan Li, Haiyan Li, Yuzhu Zhao, Peixuan Zong, Zongxiang Zhan, Zhongyun Piao   +5 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

A Defined Agar Medium for Genetic Transformation of \u3cem\u3eNeisseria meningitidis\u3c/em\u3e [PDF]

, 1962
Catlin, B. Wesley (Marquette University, Milwaukee, Wis.) and Gertrude M. Schloer. A defined agar medium for genetic transformation of Neisseria meningitidis. J. Bacteriol. 83:470–474.
Catlin, B. Wesley, Schloer, Gertrude M.
core   +1 more source

Optimization of Agrobacterium tumefaciens-mediated transformation and shoot regeneration after co-cultivation of cabbage (Brassica oleracea subsp. capitata) cv. KY Cross with AtHSP101 gene [PDF]

, 2010
A number of parameters which have been reported to influence genetic transformation via Agrobacterium-mediated transformation method were evaluated to increase the frequency of transformation of cabbage (Brassica oleracea subsp. capitata) cv.
Abdul Aziz, Maheran, Abdul Rashid, Azmi, Abdullah, Siti Nor Akmar, Kamaladini, Hossein, Nobandegani, Mohammad Bagher Javadi, Rafat, Arash, Sirchi, Mohammad Hossein Torabi   +6 more
core   +1 more source

Genetic Transformation and Complementation [PDF]

, 2017
The disciplines of Borrelia (Borreliella) burgdorferi microbiology and Lyme disease pathogenesis have come to depend on the genetic manipulation of the spirochete. Generating mutants in these recalcitrant bacteria, while not straightforward, is routinely accomplished in numerous laboratories, although there are several crucial caveats to consider. This
D Scott, Samuels, Dan, Drecktrah, Laura S, Hall   +2 more
openaire   +2 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Genetic resources and genetic transformation in bermudagrass – a review

Biotechnology & Biotechnological Equipment, 2018
Bermudagrass is widely distributed as a warm-season turf and forage grass in the temperate and tropical zones around the world. Its strong vegetative reproduction and ability to withstand drought make it an ideal species as a most used warm-season forage
Shilian Huang, Chen Wang, Junsong Liang
doaj   +1 more source

Genetic transformation of eucalyptus [PDF]

Physiology and Molecular Biology of Plants, 2011
Eucalyptus is the second most widely planted multipurpose woody tree species in the world. It is a commercially important hardwood tree for paper and wood industries. In the past two decades, various research groups reported different genetic transformation protocols and attempts towards development of transgenic eucalyptus. Much of the work related to
openaire   +2 more sources