Results 31 to 40 of about 1,712,319 (264)

Production of transgenic mice

Folia Pharmacologica Japonica, 1994
Transgenic mice are very useful for analyzing the functions of a gene either at the tissue level or at the whole body level. Here we describe the production of transgenic mice by the microinjection of DNA into the pronuclei of fertilized mouse eggs.
openaire   +5 more sources

Transgenic Mice for cGMP Imaging [PDF]

Circulation Research, 2013
Rationale: Cyclic GMP (cGMP) is an important intracellular signaling molecule in the cardiovascular system, but its spatiotemporal dynamics in vivo is largely unknown. Objective:
Martin, Thunemann, Lai, Wen, Matthias, Hillenbrand, Angelos, Vachaviolos, Susanne, Feil, Thomas, Ott, Xiaoxing, Han, Dai, Fukumura, Rakesh K, Jain, Michael, Russwurm, Cor, de Wit, Robert, Feil   +11 more
openaire   +3 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Human apoE3 but not apoE4 rescues impaired astrocyte activation in apoE null mice

Neurobiology of Disease, 2003
The allele E4 of apolipoprotein E (apoE) is an important risk factor for Alzheimer’s disease (AD) and the chronic brain inflammation which is associated with AD is more pronounced in subjects who carry this allele.
Gal Ophir, Sigal Meilin, Margalit Efrati, Joab Chapman, Dimitri Karussis, Allen Roses, Daniel M Michaelson   +6 more
doaj   +1 more source

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Enhanced UV-Induced Skin Carcinogenesis in Transgenic Mice Overexpressing Proprotein Convertases

Neoplasia: An International Journal for Oncology Research, 2013
The proprotein convertases (PCs) furin and PACE4 process numerous substrates involved in tumor growth, invasion, and metastasis. We have previously shown that PCs increase the susceptibility to chemical skin carcinogenesis. Because of the human relevancy
Jian Fu, Daniel E. Bassi, Jirong Zhang, Tianyu Li, Kathy Q. Cai, Courtney Lyons Testa, Emmanuelle Nicolas, Andres J. Klein-Szanto   +7 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Generation of H11-albumin-rtTA Transgenic Mice: A Tool for Inducible Gene Expression in the Liver

G3: Genes, Genomes, Genetics, 2019
The modification of the mouse genome by site-specific gene insertion of transgenes and other genetic elements allows the study of gene function in different developmental stages and in the pathogenesis of diseases.
Yu-Shan Li, Ran-Ran Meng, Xiu Chen, Cui-Ling Shang, Hong-Bin Li, Tao-Jun Zhang, Hua-Yang Long, Hui-Qi Li, Yi-Jing Wang, Feng-Chao Wang   +9 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source