Results 261 to 270 of about 1,635,517 (312)
Some of the next articles are maybe not open access.
2013
The generation of transgenic mouse models has been a powerful technique for several decades and is still widely used. There have been many manuals and general reviews of this technology. This chapter is designed to be a "how-to" resource with detailed specifics.
Jennifer, Skelton +3 more
openaire +2 more sources
The generation of transgenic mouse models has been a powerful technique for several decades and is still widely used. There have been many manuals and general reviews of this technology. This chapter is designed to be a "how-to" resource with detailed specifics.
Jennifer, Skelton +3 more
openaire +2 more sources
Cryopreservation of Transgenic Mouse Lines
2003A transgenic animal represents an enormous investment in time and money. Animals can be destroyed through disease, fire, malfuncnons in the control of the environment, negligence, sabotage, or accidental disposal. Researchers can protect valuable transgenic lines from accrdental destruction by "banking" them in liquid nitrogen.
Jillian M, Shaw, Naomi, Nakagata
openaire +4 more sources
Transgenic Mouse Colony Management
Current Protocols in Molecular Biology, 2005AbstractThis unit describes strategies and methods used to establish a colony of transgenic mice. Basic mating and genotyping strategies are introduced.
openaire +2 more sources
Transgenic Mouse and Gene Therapy
Diabetes, 1996In the transgenic mouse, a specific gene can be transduced or deleted to study its function and relation to human diseases. Recently, various lines of transgenic mice that overexpress or lack a specific gene have been established and are available to study the pathophysiology of human diseases, including atherosclerosis, diabetes, and hyperlipidemia ...
K, Harada +3 more
openaire +2 more sources
2009
With the development of the Cre-LoxP system, conditional gene targeting has rapidly become a powerful technology that facilitates the study of gene function. This advanced technique circumvents three major concerns sometimes levelled against conventional transgenic and gene-targeting approaches.
openaire +2 more sources
With the development of the Cre-LoxP system, conditional gene targeting has rapidly become a powerful technology that facilitates the study of gene function. This advanced technique circumvents three major concerns sometimes levelled against conventional transgenic and gene-targeting approaches.
openaire +2 more sources
Non-obese diabetic transgenic mouse
Springer Seminars in Immunopathology, 1992Several important conclusions can be drawn from these experiments and are as follows. First, not only the lack of I-E but also the presence of unique I-A are involved in the development of autoimmune insulitis in a NOD mouse. Second, the mechanism of prevention in I-Ak transgenic mice might be different from that in I-E transgenic mice, although the ...
K, Yamamura +4 more
openaire +2 more sources
1986
This chapter discusses the preparation of the transgenic mouse. Transgenic mice are generated by the introduction of a cloned piece of DNA into a fertilized mouse egg, with subsequent growth of the embryo to birth and beyond. This method allows the function of specific genes and regulatory elements to be examined in the context of the whole animal with
Leonard G. Davis +2 more
openaire +1 more source
This chapter discusses the preparation of the transgenic mouse. Transgenic mice are generated by the introduction of a cloned piece of DNA into a fertilized mouse egg, with subsequent growth of the embryo to birth and beyond. This method allows the function of specific genes and regulatory elements to be examined in the context of the whole animal with
Leonard G. Davis +2 more
openaire +1 more source
Transgenic mouse models of Alzheimer's disease
Trends in Genetics, 2001Recent advances in the understanding of the genetic basis of Alzheimer's disease have enabled the production of transgenic mouse models of the disease. Utilizing both cDNA- and genomic-based approaches, these mouse models for Alzheimer's disease have already provided valuable insights into the pathogenesis of the disease and potential therapeutic ...
B J, Hock, B T, Lamb
openaire +4 more sources
Inducible Transgenic Mouse Models
2010Inducible transgenic mouse models allow for the activation of genes in specific cells and tissues at specific times. Expression levels are dependent on the dose of the agent administered. Effective experimental models are characterized by low background levels of the regulated gene and induction to high levels with sub-physiological levels of inducing ...
openaire +2 more sources
Transgenic Mouse Model of Stunned Myocardium
Science, 2000Stunned myocardium is a syndrome of reversible contractile failure that frequently complicates coronary artery disease. Cardiac excitation is uncoupled from contraction at the level of the myofilaments. Selective proteolysis of the thin filament protein troponin I has been correlated with stunned myocardium.
A M, Murphy +6 more
openaire +2 more sources