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Transgenic Mouse and Gene Therapy
Diabetes, 1996In the transgenic mouse, a specific gene can be transduced or deleted to study its function and relation to human diseases. Recently, various lines of transgenic mice that overexpress or lack a specific gene have been established and are available to study the pathophysiology of human diseases, including atherosclerosis, diabetes, and hyperlipidemia ...
K, Harada +3 more
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Autopsy and Histological Analysis of the Transgenic Mouse
2003Over the past decades, transgenic and knock-out mouse models have become common use in research laboratories. Detailed phenotypic characterization of such models is essential for understanding basic mechanisms of normal physiology and disease. Hereto, pathological examination is a very helpful tool. This chapter describes detailed procedures to perform
Gijbels, Marion J. J. +1 more
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Transgenic RNAi Applications in the Mouse
2010Within the past 10 years, RNA interference has emerged as a powerful experimental tool as it allows rapid gene function analysis. Unique features such as reversibility of gene silencing and simultaneous targeting of several genes characterize the approach. In this chapter, transgenic RNAi techniques in reverse mouse genetics are discussed and protocols
Seibler, Jost (Prof. Dr.) +1 more
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Cloning a Transgene for Transgenic RNAi in Mouse Oocytes
Cold Spring Harbor Protocols, 2009INTRODUCTIONRNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for many model systems. The protocol presented here describes the cloning of a plasmid construct for use in transgenic RNAi experiments in mouse oocytes. The protocol is intended for production of a transgene by cloning an inverted repeat (
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2009
With the development of the Cre-LoxP system, conditional gene targeting has rapidly become a powerful technology that facilitates the study of gene function. This advanced technique circumvents three major concerns sometimes levelled against conventional transgenic and gene-targeting approaches.
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With the development of the Cre-LoxP system, conditional gene targeting has rapidly become a powerful technology that facilitates the study of gene function. This advanced technique circumvents three major concerns sometimes levelled against conventional transgenic and gene-targeting approaches.
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A Transgenic Mouse Model of Poliomyelitis
2016Transgenic mice (tg mice) that express the human poliovirus receptor (PVR), CD155, are susceptible to poliovirus and develop a neurological disease that resembles human poliomyelitis. Assessment of the neurovirulence levels of poliovirus strains, including mutant viruses produced by reverse genetics, circulating vaccine-derived poliovirus, and vaccine ...
Satoshi, Koike, Noriyo, Nagata
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An Overview of Transgenic Mouse Production
2003Three basic techniques have emerged for making transgenic mice: two of these, retroviral infection of preimplantation embryos and the manipulation of embryonal stem cells, have attributes which make them desirable in some experiments, but the third technique, microinjection, is by far the most efficient and popular.
D, Murphy, D A, Carter
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Transgenic Myth to Transgenic Mouse
1993Abstract The last few years I’ve been watching with ever more interest the research on genetic engineering that produces transgenic life-forms. Transgenic forms are the fascinating creations that result from mixing genetic material between different species.
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Transgenic Mouse Models of CMT1A and HNPP
Annals of the New York Academy of Sciences, 1999ABSTRACT: We have generated several PMP22 animal mutants with altered PMP22 gene dosage. A moderate increase in the number of PMP22 genes led to hypomyelination comparable to CMT1A, whereas high copy numbers of transgenic PMP22 resulted in phenotypes resembling more severe forms of hereditary motor and sensory neuropathies.
Suter, U., Nave, K.
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Transgenic Mouse Colony Management
Current Protocols in Molecular Biology, 2005AbstractThis unit describes strategies and methods used to establish a colony of transgenic mice. Basic mating and genotyping strategies are introduced.
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