Results 1 to 10 of about 3,961,294 (191)

Whole genome mutagenicity evaluation using Hawk-Seq™ demonstrates high inter-laboratory reproducibility and concordance with the transgenic rodent gene mutation assay [PDF]

Genes and Environment
Background Error-corrected next-generation sequencing (ecNGS) enables the sensitive detection of chemically induced mutations. Matsumura et al. reported Hawk-Seq™, an ecNGS method, demonstrating its utility in clarifying mutagenicity both qualitatively ...
Shoji Matsumura, Sayaka Hosoi, Takako Hirose, Yuki Otsubo, Kazutoshi Saito, Masaaki Miyazawa, Akihiro Kawade, Atsushi Hakura, Dai Kakiuchi, Shoji Asakura, Naoki Koyama, Yuki Okada, Satsuki Chikura, Takafumi Kimoto, Kenichi Masumura, Takayoshi Suzuki, Kei-ichi Sugiyama   +16 more
doaj   +5 more sources

Comparing BMD-derived genotoxic potency estimations across variants of the transgenic rodent gene mutation assay. [PDF]

Environ Mol Mutagen, 2017
There is growing interest in quantitative analysis of in vivo genetic toxicity dose‐response data, and use of point‐of‐departure (PoD) metrics such as the benchmark dose (BMD) for human health risk assessment (HHRA). Currently, multiple transgenic rodent (TGR) assay variants, employing different rodent strains and reporter transgenes, are used for the ...
Wills JW, Johnson GE, Battaion HL, Slob W, White PA.   +4 more
europepmc   +12 more sources

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene. [PDF]

Environ Mol Mutagen
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Göpfert A, Ronnenberg KKM, Ruelker C, Spang S, Honarvar N, Landsiedel R.   +5 more
europepmc   +4 more sources

In vivo mutagenicity assessment of styrene in MutaMouse liver and lung

Genes and Environment, 2023
Background Styrene (CAS 100-42-5) is widely used as polystyrene and acrylonitrile–butadiene–styrene resin such as plastic, rubber, and paint. One of the primary uses of styrene is food utensils and containers, but a small amount of styrene transferred ...
Yasumasa Murata, Masakatsu Natsume, Takako Iso, Yoshiyuki Shigeta, Nozomu Hirose, Takaaki Umano, Katsuyoshi Horibata, Kei-ichi Sugiyama, Kenichi Masumura, Akihiko Hirose, Mariko Matsumoto   +10 more
doaj   +1 more source

In vivo mutagenicity assessment of orally treated tert-butyl hydroperoxide in the liver and glandular stomach of MutaMouse

Genes and Environment, 2023
Background tert-Butyl hydroperoxide (TBHP; CAS 75–91-2), a hydroperoxide, is mainly used as a polymerization initiator to produce polyethylene, polyvinyl chloride, and unsaturated polyester.
Yasumasa Murata, Kenichiro Suzuki, Yoshiyuki Shigeta, Takako Iso, Nozomu Hirose, Takaaki Umano, Katsuyoshi Horibata, Kei-ichi Sugiyama, Akihiko Hirose, Kenichi Masumura, Mariko Matsumoto   +10 more
doaj   +1 more source

Duplex sequencing identifies genomic features that determine susceptibility to benzo(a)pyrene-induced in vivo mutations

BMC Genomics, 2022
Exposure to environmental mutagens increases the risk of cancer and genetic disorders. We used Duplex Sequencing (DS), a high-accuracy error-corrected sequencing technology, to analyze mutation induction across twenty 2.4 kb intergenic and genic targets ...
Danielle P. M. LeBlanc, Matthew Meier, Fang Yin Lo, Elizabeth Schmidt, Charles Valentine, Andrew Williams, Jesse J. Salk, Carole L. Yauk, Francesco Marchetti   +8 more
doaj   +1 more source

Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture

MethodsX, 2021
Successful use of the CRISPR-Cas9 system for gene manipulation relies on identifying effective and efficient guide RNA sequences (gRNAs). When the goal is to create transgenic animal/rodent models by knocking-in desired sequences using homology-directed ...
Anil K. Challa, Denise Stanford, Antonio Allen, Lawrence Rasmussen, Ferdinand K. Amanor, S. Vamsee Raju   +5 more
doaj   +1 more source

In vivo and in vitro mutagenicity of perillaldehyde and cinnamaldehyde

Genes and Environment, 2021
Background Perillaldehyde and cinnamaldehyde are natural substances found in plants that are used as flavoring ingredients. Due to the α,β-unsaturated aldehydes in their structures, these compounds are expected to be DNA reactive. Indeed, several reports
Masamitsu Honma, Masami Yamada, Manabu Yasui, Katsuyoshi Horibata, Kei-ichi Sugiyama, Kenichi Masumura   +5 more
doaj   +1 more source

Summarized data of genotoxicity tests for designated food additives in Japan

Genes and Environment, 2018
The Ministry of Health, Labour and Welfare has carried out genotoxicity tests for food additives used in Japan in cooperation with the Japan Food Additives Association since 1979. Hayashi et al.
Masami Yamada, Masamitsu Honma
doaj   +1 more source

Tissue-specific mutagenicity of N-nitroso-methylphenylamine assayed by the transgenic rodent assay and duplex sequencing

Frontiers in Toxicology
N-Nitroso-methylphenylamine (NMPA) is a rat esophageal carcinogen that has been found in food, tobacco and drugs. In this study, NMPA was administered via drinking water to Big Blue transgenic rats over 28 days following methods recommended in OECD TG ...
Li Xia, Jian Yan, Kamela Mitchell, Robert H. Heflich, Ying Chen, Timothy J. McGovern, Aisar H. Atrakchi, Tao Chen   +7 more
doaj   +1 more source