Molecular Therapy: Methods & Clinical DevelopmentThe hitherto unexplained reduction of cell-specific productivity in transient gene expression (TGE) at high cell density (HCD) is known as the cell density effect (CDE).
Pol Pérez-Rubio +5 more
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FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Applied BiosciencesProtoplasts offer a promising alternative to embryogenic cell suspensions (ECS) for gene editing in banana, potentially overcoming several limitations associated with ECS-based transformation systems. This study aimed to optimize protoplast isolation and
Pradeep Chand Deo +4 more
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Efficient Gene Transfer to Human Endothelial Cells Using DNA Complexed to Adenovirus Particles
BioTechniques, 1998 Human endothelial cells have been found to be relatively refractory to various methods of DNA transfection currently in common use. By using a transfection method involving DNA complexed with replication-deficient adenovirus particles, we have shown that
Cora-Jean S. Edgell +3 more
doaj +1 more source
Role for polo-like kinase 4 in mediation of cytokinesis. [PDF]
, 2019 The mitotic protein polo-like kinase 4 (PLK4) plays a critical role in centrosome duplication for cell division. By using immunofluorescence, we confirm that PLK4 is localized to centrosomes.
Brugge, Joan S +10 more
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Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
DNMT3B in vitro knocking-down is able to reverse embryonal rhabdomyosarcoma cell phenotype through inhibition of proliferation and induction of myogenic differentiation [PDF]
, 2016 Aberrant DNA methylation has been frequently observed in many human cancers, including rhabdomyosarcoma (RMS), the most common soft tissue sarcoma in children.
Camero, Simona +11 more
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Developmental and Epileptic Encephalopathy due to Biallelic Pathogenic Variants in PIGM
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective PIGM encodes a critical enzyme in the glycosylphosphatidylinositol (GPI)‐anchor biosynthesis pathway. While promoter‐region mutations in PIGM have been associated with a relatively mild phenotype characterized by portal vein thrombosis and absence seizures, recent evidence suggests that coding‐region mutations result in a more severe
Júlia Sala‐Coromina +11 more
wiley +1 more source
Poly(Beta-Amino Ester)s as High-Yield Transfection Reagents for Recombinant Protein Production
International Journal of Nanomedicine, 2022 Kathryn M Luly,1,2 Huilin Yang,2,3 Stephen J Lee,2,3 Wentao Wang,1,2 Seth D Ludwig,2,3 Haley E Tarbox,4 David R Wilson,1,2,5 Jordan J Green,1– 3,5– 10 Jamie B Spangler1– 3,6,8 1Department of Biomedical Engineering, Johns Hopkins University School of ...
Luly KM +8 more
doaj
The regions of the herpes simplex virus type 1 immediate early protein Vmwl75 required for site specific DNA binding closely correspond to those involved in transcriptional regulation [PDF]
, 1988 The immediate-early (IE) protein Vmw175 (ICP4) of HSV-1 is required for the transcription of later classes of viral genes and the repression of IE gene expression.
Everett, R.D., Paterson, T.
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