Results 181 to 190 of about 270,633 (290)

Delivery of Pleckstrin‐Homology Domains Suppresses PI3K/Akt Signaling and Breast Cancer Metastasis

Advanced Science, EarlyView.
Current therapies curb tumor growth but not metastasis. Obscurin, a giant metastasis suppressor lost in breast cancer, restrains PI3K/Akt signaling but is impractical to restore. We deploy a mini‐obscurin, comprising the obscurin‐PH‐domain, which sequesters PI3K‐p85, potently suppressing invasion and metastasis.
Matthew Eason, Anindya Sen, Suhan Cho, Se Jong Lee, Keyata Thompson, Md Musavvir Mahmud, Poornima Dubey, Talia Guardia, Anthony Kim, Stuart Martin, Nathan Wright, Konstantinos Konstantopoulos, Aikaterini Kontrogianni‐Konstantopoulos   +12 more
wiley   +1 more source

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing. [PDF]

Biotechnol Bioeng
Green EA, Fu Q, Ndahiro N, Leibiger TM, Wang Y, Lee Y, Lee KH, Betenbaugh M, Yoon S, McNally DJ.   +9 more
europepmc   +1 more source

High cell density transient transfection of CHO cells for TGF-β1 expression. [PDF]

Eng Life Sci, 2019
Elshereef AA, Jochums A, Lavrentieva A, Stuckenberg L, Scheper T, Solle D.   +5 more
europepmc   +1 more source

Reduced Maintenance DNA Methylation Thresholds Enable Sensitive Reporter Assays for UHRF1 and DNMT1 Inhibition

Advanced Science, EarlyView.
The authors engineer colorectal cancer cells with reduced redundancy of UHRF1 and DNMT1, key DNA methylation maintenance factors, lowering the maintenance threshold and sensitizing cells to inhibition. They develop reporter assays driven by endogenous tumor‐suppressor gene promoters with enhanced sensitivity and dynamic range to demethylating drugs ...
Cuicui Xia, Ying Cui, Jiongliang Wang, Wenjing Bai, Danyang Wang, Yao Chen, Huahui Guo, Liangyi Zong, Bing Qin, Xintong Cai, Jie Wang, Jing Huang, Scott B. Rothbart, Zufeng Guo, Stephen B. Baylin, Xiangqian Kong   +15 more
wiley   +1 more source

Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells. [PDF]

Biotechnol Lett
Pistek M, Andorfer P, Grabherr R, Kraus B, Hernandez Bort JA.   +4 more
europepmc   +1 more source

Activation of ERBB4 Pathway Inhibits Pathological Transdifferentiation of Lung Epithelial Progenitors into CD66c+ Basal Cells in Severe Lung Injury

Advanced Science, EarlyView.
In fibrotic distal lung regions, CD66c+ basal cells emerge as a pathological state. Using human distal lung organoids, this study identifies CD66c+ basal cells as a pro‐fibrotic state arising through transdifferentiation from secretory, AT2, and basal cells.
Kaijun Lin, Xinran Deng, Haonan Wang, Yamei Jiang, Jian Sun, Hailin Ding, Ming Ye, Xiaoting Wang, Yu Wang, Li Yuan, Zhenju Song, Xinhua Lin, Shenfei Sun, Ning Jiang   +13 more
wiley   +1 more source

Improving Transient Transfection Efficiency in a Differentiated, Polar Epithelial Cell Layer. [PDF]

J Biomol Tech, 2019
Rybakovsky E, Valenzano MC, DiGuilio KM, Buleza NB, Moskalenko DV, Harty RN, Mullin JM.   +6 more
europepmc   +1 more source

Myeloid‐Derived Grancalcin Promotes Periodontal Inflammation and Pathological Bone Remodeling in Periodontitis

Advanced Science, EarlyView.
Grancalcin (GCA), a myeloid‐derived protein, is enriched in gingival tissues of periodontitis patients and mouse models. Through interactions with CD44 and activation of MYH9, GCA promotes NF‐κB signaling and exacerbates periodontal inflammation and bone loss.
Min Zhou, Yao Xiao, Ziyue Xu, Chengyu Yang, Jiang Li, Ya Liu, Ping Yin   +6 more
wiley   +1 more source

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection. [PDF]

PLoS One
Mousavi Kahaki SA, Ebrahimzadeh N, Fahimi H, Moshiri A.   +3 more
europepmc   +1 more source

Development of a high efficient promoter finding method based on transient transfection. [PDF]

Gene X, 2019
Lu Y, Li Q, Zheng K, Fu C, Jiang C, Zhou D, Xia C, Ma S.   +7 more
europepmc   +1 more source