Results 81 to 90 of about 9,270 (176)

Evidence of efficient stop codon readthrough in four mammalian genes [PDF]

, 2014
Stop codon readthrough is used extensively by viruses to expand their gene expression. Until recent discoveries in Drosophila, only a very limited number of readthrough cases in chromosomal genes had been reported.
Atkins, John F., Baranov, Pavel V., Chou, Ming-Yuan, Ivanov, Ivaylo P., Jungreis, Irwin, Kellis, Manolis, Kiran, Anmol M., Loughran, Gary   +7 more
core   +3 more sources

Metamorphosis and lncRNAs: A Close Relationship

genesis, Volume 64, Issue 2, April 2026.
ABSTRACT The classical definition of metamorphosis is a post‐embryonic transformation, such as from a tadpole to a froglet. However, recent studies suggest this process occurs to some degree in all vertebrates, as the underlying endocrine and molecular pathways are highly conserved. With the advent of high‐throughput sequencing, transcriptomic data for
H. Herrera‐Orozco, H. A. Pérez‐Mendoza   +1 more
wiley   +1 more source

Distal nucleotides affect the rate of stop codon read‐through

Quantitative Biology, 2023
Background A key step in gene expression is the recognition of the stop codon to terminate translation at the correct position. However, it has been observed that ribosomes can misinterpret the stop codon and continue the translation in the 3′UTR region.
Luciana I. Escobar, Andres M. Alonso, Jorge R. Ronderos, Luis Diambra   +3 more
doaj   +1 more source

An Engineered Nonsense \u3cem\u3eURA3\u3c/em\u3e Allele Provides a Versatile System to Detect the Presence, Absence and Appearance of the [em\u3ePSI\u3c/em\u3e\u3csup\u3e+\u3c/sup\u3e] Prion in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e [PDF]

, 2006
Common methods to identify yeast cells containing the prion form of the Sup35 translation termination factor, [PSI+], involve a nonsense suppressor phenotype. Decreased function of Sup35p in [PSI+] cells leads to readthrough of certain nonsense mutations
Kirkland, Kathryn T., Liebman, Susan W., Manogaran, Anita L.   +2 more
core   +1 more source

A conserved and essential basic region mediates tRNA binding to the Elp1 subunit of the Saccharomyces cerevisiae Elongator complex [PDF]

, 2014
Elongator is a conserved, multi-protein complex discovered in Saccharomyces cerevisiae, loss of which confers a range of pleiotropic phenotypes. Elongator in higher eukaryotes is required for normal growth and development and a mutation in the largest ...
Amberg D.C., Butler A.R., Kishida M., Sambrook J., Sikorski R.S., Tyson C.B.   +5 more
core   +4 more sources

Accessing intractable, phosphorylated intrinsically disordered proteins via a protease‐cleavable inclusion body tag

Protein Science, Volume 35, Issue 4, April 2026.
Abstract Phosphorylated intrinsically disordered proteins (IDPs), such as Bcl2‐associated agonist of cell death (BAD) and Tau, play critical roles in apoptosis and neurodegeneration, yet their instability during recombinant expression due to degradation, aggregation, and dephosphorylation, has limited structural and mechanistic studies.
Cat Hoang Vesely, Stanislau Stanisheuski, Edward Lien, Patrick Reardon, Peter J. Chung, Ryan A. Mehl, Richard B. Cooley   +6 more
wiley   +1 more source

Stops making sense: translational trade-offs and stop codon reassignment

BMC Evolutionary Biology, 2011
Background Efficient gene expression involves a trade-off between (i) premature termination of protein synthesis; and (ii) readthrough, where the ribosome fails to dissociate at the terminal stop.
Elgar Greg S, Lichtenstein Conrad P, Cotton James A, Johnson Louise J, Nichols Richard A, Polly p David, Le Comber Steven C   +6 more
doaj   +1 more source

The Readthrough Isoform AQP4ex Is Constitutively Phosphorylated in the Perivascular Astrocyte Endfeet of Human Brain

Biomolecules, 2022
AQP4ex is a recently discovered isoform of AQP4 generated by a translational readthrough mechanism. It is strongly expressed at the astrocyte perivascular endfeet as a component of the supramolecular membrane complex, commonly called orthogonal array of ...
Roberta Pati, Claudia Palazzo, Onofrio Valente, Pasqua Abbrescia, Raffaella Messina, Nicoletta Concetta Surdo, Konstantinos Lefkimmiatis, Francesco Signorelli, Grazia Paola Nicchia, Antonio Frigeri   +9 more
doaj   +1 more source

When R‐Loops Go Awry: Genome Instability and Neurological Diseases

European Journal of Neuroscience, Volume 63, Issue 8, April 2026.
DNA normally exists as a double helix formed by two complementary strands. During gene transcription, however, one strand of DNA can bind to RNA, causing the other DNA strand to be displaced. This creates a structure called an R‐loop. R‐loops play important roles in normal cellular processes such as gene expression, DNA replication, and transcription ...
Nur Rasyiqin Rasli, Yu Katsuyama
wiley   +1 more source

Aminoglycoside-mediated promotion of translation readthrough occurs through a non-stochastic mechanism that competes with translation termination [PDF]

Human Molecular Genetics, 2017
Attempts have been made to treat nonsense-associated genetic disorders by chemical agents and hence an improved mechanistic insight into the decoding of readthrough signals is essential for the identification and characterisation of factors for the treatment of these disorders.
Chowdhury, H.M., Siddiqui, M.A., Kanneganti, S., Sharmin, N., Chowdhury, M.W., Nasim, Md. Talat   +5 more
openaire   +3 more sources