Results 151 to 160 of about 149,269 (182)
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Trends in Plant Science, 2002
Mutator (Mu) element insertion has become the main way of mutating and cloning maize genes, but we are only beginning to understand how this transposon system is regulated. Mu elements are under tight developmental control and are subject to a form of epigenetic regulation that shares some features with the regulation of paramutable maize genes.
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Mutator (Mu) element insertion has become the main way of mutating and cloning maize genes, but we are only beginning to understand how this transposon system is regulated. Mu elements are under tight developmental control and are subject to a form of epigenetic regulation that shares some features with the regulation of paramutable maize genes.
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Annual Review of Microbiology, 1993
The bacterial transposon Tn5 encodes two proteins, the transposase and a related protein, the transposition inhibitor, whose relative abundance determines, in part, the frequency of Tn5 transposition. The synthesis of these proteins is programmed by a complex set of genetic regulatory elements.
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The bacterial transposon Tn5 encodes two proteins, the transposase and a related protein, the transposition inhibitor, whose relative abundance determines, in part, the frequency of Tn5 transposition. The synthesis of these proteins is programmed by a complex set of genetic regulatory elements.
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Transposon Display: A Versatile Method for Transposon Tagging
2013Transposon tagging has been used successfully in a range of organisms for the cloning of mutants of interest. In species containing high copy numbers of transposable elements combined with a high transposition rate, forward cloning can be quite challenging and requires specific high-resolution methods.
Vandenbussche, Michiel +2 more
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2014
Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
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Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
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Science (New York, N.Y.), 2010
Ac (Activator) is the first autonomous transposon described by McClintock. Its ability to create DSBs (Double Strand Breaks) makes it a powerful mutagen. In the presence of the Ac transposase, its non-autonomous Ds (Dissociation) counterparts are just as mutagenic.
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Ac (Activator) is the first autonomous transposon described by McClintock. Its ability to create DSBs (Double Strand Breaks) makes it a powerful mutagen. In the presence of the Ac transposase, its non-autonomous Ds (Dissociation) counterparts are just as mutagenic.
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Cold Spring Harbor Protocols, 2016
Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon “calling cards,” in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to
David, Mayhew, Robi D, Mitra
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Identifying the genomic targets of transcription factors is an important step in understanding the regulatory networks of gene transcription in yeast. We have developed a method that utilizes what we refer to as transposon “calling cards,” in which a transcription factor directs the Ty5 retrotransposase to insert transposons into the genome adjacent to
David, Mayhew, Robi D, Mitra
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Trends in Ecology & Evolution, 1998
In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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2009
Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication.
M Sloan, Siegrist, Eric J, Rubin
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Phage transduction is an attractive method of genetic manipulation in mycobacteria. PhiMycoMarT7 is well suited for transposon mutagenesis as it is temperature sensitive for replication and contains T7 promoters that promote transcription, a highly active transposase gene, and an Escherichia coli oriR6 K origin of replication.
M Sloan, Siegrist, Eric J, Rubin
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Bacterial catabolic transposons
Applied Microbiology and Biotechnology, 1999The introduction of foreign organic hydrocarbons into the environment in recent years, as in the widespread use of antibiotics, has resulted in the evolution of novel adaptive mechanisms by bacteria for the biodegradation of the organic pollutants. Plasmids have been implicated in the catabolism of many of these complex xenobiotics. The catabolic genes
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