Results 81 to 90 of about 111,214 (295)
, 2013 The success of gene transfer has been demonstrated in many of vertebrate species, whereas the efficiency of producing transgenic animals remains pretty low due to the random integration of foreign genes into a recipient genome.
Long, Yong +8 more
core +1 more source
Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis [PDF]
, 2019 While transposon mutagenesis has been developed for Mycobacterium avium subspecies paratuberculosis (Map), relatively few laboratories have adopted this important genetic tool to examine gene function and essentiality.
Bannantine, John P. +5 more
core +1 more source
Advanced Science, EarlyView.Streptococcal mannose phosphotransferase system component IID (Man‐PTSIID) is identified as a novel RANK‐binding osteoclastogenic factor. By directly binding to RANK and activating NF‐κB independently of TLR2, Man‐PTSIID drives osteoclastogenesis and inflammatory bone destruction, uncovering an unexpected microbial mechanism underlying streptococcal ...
Chaeyeon Park +13 more
wiley +1 more source
A Synthetic Platform for Antibody Junctional Diversification Beyond Natural Constraints
Advanced Science, EarlyView.ARESEC is a synthetic platform that reconstructs functional V(D)J recombination in HEK293T cells. By introducing RSS elements and co‐expressing RAG1/2 and TdT across all three antibody CDRs, it enables programmable junctional diversification and direct generation of high‐affinity variants targeting PD‐1/PD‐L1, overcoming the evolutionary constraints of
Wang Liu +3 more
wiley +1 more source
Mapping the transposon insertion sites.
, 2018 (A) Diagram showing the insertion of the transposon cassette into a chromosomal region (in grey). Primers specific to the transposon ends were paired with universal primers to amplify the chromosome- transposon junctions (red triangles), using semi ...
Rebecca L. Lamason (322448) +2 more
core +1 more source
CAR‐Engineered Cell Therapies Beyond Cancer: Reprogramming Fibrosis and Immune‐Mediated Inflammation
Advanced Science, EarlyView.CAR‐engineered cell therapies are expanding beyond cancer toward immune resetting, pathological‐cell clearance, matrix remodeling, and microenvironmental reprogramming in autoimmune, inflammatory, and fibrotic diseases. This Review compares CAR‐T, CAR‐macrophage, and CAR‐NK platforms and proposes controllable spatiotemporal reprogramming to align ...
Peng Jun Xu +6 more
wiley +1 more source
Advanced Science, EarlyView.By integrating 516 whole‐genome resequencing datasets and 236 transcriptomes of allotetraploid common carp, this study establishes the first population‐scale atlas of transposable element (TE) variation in teleost species. TE bursts, relaxed purifying selection, and lineage‐specific loss of ancient insertions shape genome evolution and phenotypic ...
Shuimu Hu +11 more
wiley +1 more source
Spread of Streptococcus suis Sequence Type 7, China
Emerging Infectious Diseases, 2008 Streptococcus suis sequence type (ST) 7 has been spreading throughout China. To determine events associated with its emergence, we tested 114 isolates.
Changyun Ye +15 more
doaj +1 more source
Construction and function validation of inducible immortalized gene integration vectors
Zhongguo shuxue zazhi[Objective] To construct inducible immortalization gene vectors for transfection into primary cells, enabling the establishment of a conditionally immortalized cell line that support their sustained cultivation and proliferation in vitro. [Methods] Using
YUE Wei +4 more
doaj +1 more source
Frontiers in Microbiology, 2018 Differential fluorescent labeling of bacteria has become instrumental for many aspects of microbiological research, such as the study of biofilm formation, bacterial individuality, evolution, and bacterial behavior in complex environments.
Rudolf O. Schlechter +15 more
doaj +1 more source