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Biodegradation, 1994
The structure and function of transposable elements that code for catabolic pathways involved in the biodegradation of organic compounds are reviewed. Seven of these catabolic transposons have structural features that place them in the Class I (composite) or Class II (Tn3-family) bacterial elements. One is a conjugative transposon.
R C, Wyndham +3 more
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The structure and function of transposable elements that code for catabolic pathways involved in the biodegradation of organic compounds are reviewed. Seven of these catabolic transposons have structural features that place them in the Class I (composite) or Class II (Tn3-family) bacterial elements. One is a conjugative transposon.
R C, Wyndham +3 more
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Trends in Ecology & Evolution, 1998
In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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In his interesting commentary on transposable elements (TEs), gene silencing and macroevolution, John McDonald[1xMcDonald, J. Trends Ecol. Evol. 1998; 13: 94–95Abstract | Full Text PDF | PubMed | Scopus (52)See all References[1]omitted a major player that has acted in concert with TEs to drive vertebrate and plant evolution, namely, polyploidy.
M A, Matzke, A J, Matzke
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Annual Review of Microbiology, 1993
The bacterial transposon Tn5 encodes two proteins, the transposase and a related protein, the transposition inhibitor, whose relative abundance determines, in part, the frequency of Tn5 transposition. The synthesis of these proteins is programmed by a complex set of genetic regulatory elements.
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The bacterial transposon Tn5 encodes two proteins, the transposase and a related protein, the transposition inhibitor, whose relative abundance determines, in part, the frequency of Tn5 transposition. The synthesis of these proteins is programmed by a complex set of genetic regulatory elements.
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Transposon Display: A Versatile Method for Transposon Tagging
2013Transposon tagging has been used successfully in a range of organisms for the cloning of mutants of interest. In species containing high copy numbers of transposable elements combined with a high transposition rate, forward cloning can be quite challenging and requires specific high-resolution methods.
Vandenbussche, Michiel +2 more
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DNA Sequencing with Transposons
Journal of Computational Biology, 2000The use of transposons offers the possibility of a directed approach to DNA sequencing, where a target DNA up to about 6kb in length can be sequenced quickly and with minimal redundancy. Transposons are mobile DNA elements which can be inserted in a reasonably random fashion into the target DNA.
Simon Cawley, Terence P. Speed
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CRISPR transposons on the move
Cell Host & Microbe, 2021CRISPR transposons (CASTs) represent unique mobile genetic elements that co-opted CRISPR-Cas immune systems for RNA-guided DNA transposition. However, CAST-encoded CRISPR arrays rarely match the CAST's chromosomal location. A recent publication in Cell helps resolve this paradox by revealing CRISPR-array-independent mechanisms of chromosomal homing ...
Ioannis, Mougiakos, Chase L, Beisel
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Trends in Plant Science, 2002
Mutator (Mu) element insertion has become the main way of mutating and cloning maize genes, but we are only beginning to understand how this transposon system is regulated. Mu elements are under tight developmental control and are subject to a form of epigenetic regulation that shares some features with the regulation of paramutable maize genes.
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Mutator (Mu) element insertion has become the main way of mutating and cloning maize genes, but we are only beginning to understand how this transposon system is regulated. Mu elements are under tight developmental control and are subject to a form of epigenetic regulation that shares some features with the regulation of paramutable maize genes.
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2014
Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
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Transposon-based mutagenesis of bacterial genomes is a powerful method to identify genetic elements that control specific phenotypes. The most frequently used transposon tools in Pseudomonas aeruginosa are based either on Himar1 mariner or Tn5 transposases, both of which have been used to generate nonredundant mutant libraries in P. aeruginosa. Here we
openaire +2 more sources