Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
GeographiesThe COVID-19 pandemic profoundly affected people’s travel behavior and travel desires, particularly regarding trips to prominent destinations. This study explores the pandemic’s impact on travel behavior and online search patterns for 12 landmarks across
Jiping Cao +3 more
doaj +1 more source
Investigating influencing factors for ICT adoption that changes travel behavior in response to the COVID-19 outbreak in Indonesia. [PDF]
Case Stud Transp Policy, 2023 Rizki M +5 more
europepmc +1 more source
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Digital Pathways to Sustainability: Eco-Travel Apps and Gen Z’s Eco-Friendly Travel Behaviors
Tourism and HospitalityThis study examines the interrelations among the perception of Gen Z towards tourism’s negative environmental impacts, adoption intentions of eco-friendly travel apps, and attitudes towards the value of these apps, as well as the mediating roles of ...
Zehra Saltik +2 more
doaj +1 more source
Subjective variables in travel behavior models: a critical review and Standardized Transport Attitude Measurement Protocol (STAMP). [PDF]
Transportation (Amst), 2022 Bhagat-Conway MW +5 more
europepmc +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Understanding individual-level travel behavior changes due to COVID-19: Trip frequency, trip regularity, and trip distance. [PDF]
Cities, 2023 Lee S, Ko E, Jang K, Kim S.
europepmc +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source