Results 211 to 220 of about 26,185 (255)
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Immunochemical assay of rat postheparin plasma triacylglycerol lipases
Atherosclerosis, 1980Immunochemical methods for selective measurement of lipoprotein lipase and hepatic lipase activities in rat postheparin plasma are described and validated. Lipoprotein lipase was measured using a substrate containing 10% serum and 0.1 M NaCl after inactivation of hepatic lipase with a specific antiserum. Hepatic lipase was measured at 1.0 M NaCl with a
T, Kuusi, C, Ehnholm, E A, Nikkilä
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Rapid exchange of pancreatic lipase between triacylglycerol droplets
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2004Two types of experiments were performed to study the reversibility of interfacial adsorption of pancreatic lipase (PL) to fat droplets during lipolysis. Lipolysis was measured in olive oil/gum arabic emulsions containing radiolabeled triolein in the presence of bile salts and lecithin at rate-limiting concentrations of porcine PL (PPL) or human PL (HPL)
Horst, Haiker +3 more
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Triacylglycerol Lipases in Adrenals and Testes of Rats
The Journal of Biochemistry, 1982Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column.
T, Murase +3 more
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Triacylglycerol lipase activity in the rabbit renal medulla
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1987Although the renal medulla is rich in triacylglycerols, the lipolysis of these intracellular triacylglycerols by a renomedullary triacylglycerol lipase has not been directly demonstrated. The present study demonstrates triacylglycerol lipase activity localized in the particulate subcellular fractions of rabbit renal medullae.
T E, Liston, A, Nasjletti
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Some properties of triacylglycerol lipase in chicken erythrocytes
International Journal of Biochemistry, 1986Membrane-bound acid lipase was found in the chicken erythrocytes ghosts, having an optimum pH of 4.5. The membrane-bound lipase showed its maximum activity at 38 degrees C, and it was stable below 45 degrees C. The bound lipase was activated by octyl glucoside and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), but it was markedly ...
M, Fujii +3 more
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Magnetic Resonance in Medicine, 1989
AbstractTriacylglycerol lipse activity was studied using glycerol [1‐13C] trioctanoate mixed with postheparin rat plasma. 13C NMR spectroscopy demonstrated triacylglycerol hydrolysis into free fatty acids with no difference at the 1,3 or 2 glycerol positions. There was no inhibition by high sodium concentration, consistent with lipase of hepatic origin.
R J, Dimand, E M, Bradbury, K L, Cox
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AbstractTriacylglycerol lipse activity was studied using glycerol [1‐13C] trioctanoate mixed with postheparin rat plasma. 13C NMR spectroscopy demonstrated triacylglycerol hydrolysis into free fatty acids with no difference at the 1,3 or 2 glycerol positions. There was no inhibition by high sodium concentration, consistent with lipase of hepatic origin.
R J, Dimand, E M, Bradbury, K L, Cox
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Triacylglycerol Lipase in Developing Rat Sciatic Nerve
Journal of Neurochemistry, 1986Abstract: Triacylglycerol lipase activity, with a pH optimum of 7.5, was demonstrated in cell‐free homogenates of rat sciatic endoneurium. 1,2‐Diacylglycerol was the major product of triacylglycerol hydrolysis. A rapid decline in lipase activity was found in rats up to 2 months of age. After this time, the decrease continued, but at a much slower rate.
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Regioselective analysis of triacylglycerols by lipase hydrolysis
Journal of the American Oil Chemists' Society, 1995AbstractA modified procedure for the regiospecific analysis of triacylglycerols (TAG) with a 1,3‐specific lipase is described. After partial lipase hydrolysis of the triacylglycerol, the released free fatty acids (FFA) and 1,2(2,3)‐diacylglycerols (DAG) were isolated by thin‐layer chromatography (TLC) and converted to fatty acid methyl esters (FAME ...
Thomas A. Foglia +2 more
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Triacylglycerol lipase activity in baker's yeast (Saccharomyces cerevisiae)
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976An investigation of intracellular triacylglycerol lipase activity in baker's yeast (Saccharomyces cerevisiae) has been performed using emulsified triolein as substrate. Bovine serum has been used as emulsifier since it was found superior to gum arabic and albumin with respect to reproducibility of both triacylglycerol concentration in the assay mixture
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Effect of cholera toxin on triacylglycerol lipase activity and triacylglycerol content of rat heart
Canadian Journal of Physiology and Pharmacology, 1987This study was performed to reexamine the effect of cholera toxin on total and intracellular alkaline lipoprotein lipase (LPL) activity in rat heart. In addition, the relationship between intracellular triacylglycerol (TG)lipase activity and TG content of cardiac tissue was determined in cholera toxin treated rats. One intravenous injection of cholera
W C, Miller +3 more
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