Results 241 to 250 of about 24,885 (251)
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Triacylglycerol hydrolysis by lipase in a flat membrane bioreactor
Biotechnology Techniques, 1994Hydrolysis of olive oil into fatty acids by lipase in a flat membrane module is reported. Lipase was immobilized by adsorption onto a hydrophilic cellulose acetate membrane. Hydrolysis was carried out by circulating pure olive on the enzyme side of the membrane and water phase on the other side. Conversion reached 85 % after 50 hours reaction time.
L. Szabó +3 more
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Hydrolysis of triacylglycerol emulsions by lingual lipase a microscopic study
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982The effect of lingual lipase on four different triacylglycerol emulsions was observed by light microscopy at pH 5-6. The extent of hydrolysis on the microscope slide was determined with the aid of radioactive emulsions or by analyzing the products by gas-liquid chromatography.
Margit Hamosh +4 more
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Rapid exchange of pancreatic lipase between triacylglycerol droplets
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2004Two types of experiments were performed to study the reversibility of interfacial adsorption of pancreatic lipase (PL) to fat droplets during lipolysis. Lipolysis was measured in olive oil/gum arabic emulsions containing radiolabeled triolein in the presence of bile salts and lecithin at rate-limiting concentrations of porcine PL (PPL) or human PL (HPL)
Horst Haiker +3 more
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Characterization of triacylglycerol lipase activity in human amniotic fluid
American Journal of Obstetrics and Gynecology, 1980A radiochemical assay was used to measure the triacylglycerol lipase activity found in normal human amniotic fluid at term. Enzyme activity was characterized in a partially purified extract of amniotic fluid and was found to be optimal at pH 8.0 +/- 0.2 in the presence of 5mM sodium taurocholate, with the use of emulsified tri-[3H]oleoyl glycerol as ...
Henri Ruf +3 more
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Acid triacylglycerol lipase from bovine thyroid gland
Lipids, 1978AbstractAn acid lipase has been detected in bovine thyroid tissue using triolein as a substrate. The activity, probably associated with the lysosomes, displays a rather broad pH‐optimum in the pH 4 to pH 6.5 range. The lipase activity can be partially purified by cosedimentation with lysosomes followed by solubilization through detergent and ...
M. De Wolf +3 more
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Triacylglycerol lipase activity in baker's yeast (Saccharomyces cerevisiae)
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1976An investigation of intracellular triacylglycerol lipase activity in baker's yeast (Saccharomyces cerevisiae) has been performed using emulsified triolein as substrate. Bovine serum has been used as emulsifier since it was found superior to gum arabic and albumin with respect to reproducibility of both triacylglycerol concentration in the assay mixture
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Purification and properties of a triacylglycerol lipase from Mycobacterium phlei
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1977In order to study the metabolism of triacylglycerol in mycobacteria, an intracellular particulate triacylglycerol lipase (EC 3.1.1.3) was purified 800-fold from stationary phase cells of Mycobacterium phlei. Extraction of whole cell suspensions with 5% Triton X-100, followed by ion-exchange chromatography of the extract on two successive DEAE-cellulose
John L. Paznokas, Arnold Kaplan
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A spectrophotometric assay for lipase activity utilizing immobilized triacylglycerols
Journal of Biochemical and Biophysical Methods, 1991New substrates for the determination of lipase activity have been developed. Triacylglycerols were immobilized by adsorption on an appropriate carrier or adsorbent yielding a lipase substrate in a powder form. The adsorbed triacylglycerols were easily hydrolyzed by lipases present in a reaction mixture.
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Conversion of a Carboxylesterase into a Triacylglycerol Lipase by a Random Mutation
Angewandte Chemie, 2005Julio Polaina +9 more
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Lipolysis and neutral triacylglycerol lipase activity in triacylglycerol enriched cardiac myocytes*
Journal of Molecular and Cellular Cardiology, 1984I. Ramirez +2 more
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