Results 141 to 150 of about 6,844 (179)
Some of the next articles are maybe not open access.
Cellulose hydrolysis by immobilized Trichoderma reesei cellulase
Biotechnology Letters, 2009Cellulose hydrolysis by immobilized Trichoderma reesei cellulase in the presence of a low viscosity ionic liquid, 1-ethyl-3-methylimidazolium diethyl phosphate (EMIM-DEP), was investigated. Preparation of the carrier-free immobilized cellulase was optimized with respect to concentration of the cross-linker and the type of precipitant. The addition of 2%
Paetrice O, Jones +1 more
openaire +2 more sources
Two major xylanases of Trichoderma reesei
Enzyme and Microbial Technology, 1992The two main xylanases produced by Trichoderma reesei were purified to electrophoretic homogeneity by ion-exchange and gel chromatography. The enzymes had isoelectric points of 9.0 and 5.5 and their molecular masses were 20 and 19 kDa, respectively.
Tenkanen, Maija +2 more
openaire +1 more source
Kinetics of protein secretion in Trichoderma reesei
1996In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma.
Pakula, Tiina +5 more
openaire +3 more sources
Induction of Cellulases in Trichoderma reesei
1983Various synthetic derivatives of glucose and several different disaccharides were shaken with washed mycelia of Trichoderma reesei QM 9414 to test for CM-cellulase and for β-glucosidase activity. The extent of induction always increased with increases in the concentration of the inducers. Lactose and sophorose were good inducers, the latter being about
Hans-Peter Hohn, Hermann Sahm
openaire +1 more source
Open the Pores: Electroporation for the Transformation of Trichoderma reesei
2020During the electroporation of T. reesei, linearized exogenous DNA is absorbed into swollen conidia by an electrical impulse. The advantage of this method is that it is less time-consuming, less expensive, and easier to perform than the classical protoplast transformation while at the same time having a comparable efficiency.
openaire +2 more sources
Proteomics studies in Trichoderma reesei
2002Proteome analysis is the simultaneous examination of a large subset of the protein species in a given cell. Traditionally proteomics is performed by two-dimensional gel electrophoresis combined with protein identification by mass spectrometry. We have set up proteomics methodology for the analysis of both extracellular and intracellular proteins of ...
Pakula, Tiina +2 more
openaire +2 more sources
การผลิตไซแลนเนสจาก Trichoderma reesei โดยใช้วัสดุเหลือใช้จากการเกษตร
เมื่อนำ Trichoderma reesei Rut C-30 เลี้ยงในอาหารสูตร production medium ที่ pH 5 อุณหภูมิ 30 องศาเซลเซียส โดยใช้วัสดุเหลือใช้จากการเกษตร 4 ชนิด คือรำข้าวสาลี รำข้าวเจ้า เปลือกถั่วลิสง และชานอ้อย เป็นแหล่งคาร์บอน พบว่ารำข้าวสาลีให้แอคติวิตีของไซแลนเนสสูงสุด (23.931 U/ml) และรำข้าวเจ้ามีแอคติวิตีของเซลลูเลสสูงสุด (0.656 U/ml) จากนั้นนำวัสดุเหลือใช ...openaire +1 more source
Proteome analysis in Trichoderma reesei
2004We have set up methods for intracellular and extracellular proteome analysis in Trichoderma. These include analysis of steady state total protein pattern using 2D gel electrophoresis as well as detection of actively synthesised proteins using metabolic labelling.
Pakula, Tiina +8 more
openaire +1 more source
The arabinolytic pathway in Trichoderma reesei
2004One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates ...
Aro, Nina +2 more
openaire +1 more source