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Kinetics of protein secretion in Trichoderma reesei

1996
In order to obtain knowledge of the protein secretion process in filamentous fungi, and to study the rate-limiting steps in protein production, we have developed methodology for measuring the kinetics of protein production and secretion by metabolic labelling in Trichoderma.
Pakula, Tiina   +5 more
openaire   +3 more sources

การผลิตไซแลนเนสจาก Trichoderma reesei โดยใช้วัสดุเหลือใช้จากการเกษตร

เมื่อนำ Trichoderma reesei Rut C-30 เลี้ยงในอาหารสูตร production medium ที่ pH 5 อุณหภูมิ 30 องศาเซลเซียส โดยใช้วัสดุเหลือใช้จากการเกษตร 4 ชนิด คือรำข้าวสาลี รำข้าวเจ้า เปลือกถั่วลิสง และชานอ้อย เป็นแหล่งคาร์บอน พบว่ารำข้าวสาลีให้แอคติวิตีของไซแลนเนสสูงสุด (23.931 U/ml) และรำข้าวเจ้ามีแอคติวิตีของเซลลูเลสสูงสุด (0.656 U/ml) จากนั้นนำวัสดุเหลือใช ...
openaire   +1 more source

Proteomics studies in Trichoderma reesei

2002
Proteome analysis is the simultaneous examination of a large subset of the protein species in a given cell. Traditionally proteomics is performed by two-dimensional gel electrophoresis combined with protein identification by mass spectrometry. We have set up proteomics methodology for the analysis of both extracellular and intracellular proteins of ...
Pakula, Tiina   +2 more
openaire   +2 more sources

Proteome analysis in Trichoderma reesei

2004
We have set up methods for intracellular and extracellular proteome analysis in Trichoderma. These include analysis of steady state total protein pattern using 2D gel electrophoresis as well as detection of actively synthesised proteins using metabolic labelling.
Pakula, Tiina   +8 more
openaire   +1 more source

The arabinolytic pathway in Trichoderma reesei

2004
One of the most widespread pentose sugars found in plant biomass is L-arabinose, a constituent of plant cell-wall polysaccharides L-arabinan, arabinogalactans and arabinoxylans. The pentose catabolic pathways are relevant for micro-organisms living on decaying plant material but also in biotechnology when cheap raw materials such as plant hydrolysates ...
Aro, Nina   +2 more
openaire   +1 more source

The Post-genomic Era of Trichoderma reesei : What's Next?

Trends in Biotechnology, 2016
Rafael Silva-Rocha   +2 more
exaly  

Trichoderma reesei cellulases in processing of cotton: Dissertation

2002
Today the use of enzymes in textile processing and after-care is already well established industrial technology. Enzymatic process applications have increased substantially due to developments in genetic engineering, as specific enzymes can be efficiently modified for targeted applications.
openaire   +2 more sources

The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30

Enzyme and Microbial Technology, 2000
J M S Cabral   +2 more
exaly  

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