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Transcriptomics in Trichoderma reesei
2020Transcriptomics is a powerful technique to study gene expression. The main purpose of transcriptome studies in the filamentous fungus Trichoderma reesei is the analysis of differentially expressed genes as a transcriptional response of the genome to different environmental stimuli or physiological conditions such as sugar availability, nitrogen ...
Amanda C C, Antonieto, Roberto N, Silva
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On the safety of Trichoderma reesei
Journal of Biotechnology, 1994Trichoderma reesei has a long history of safe use in industrial-scale enzyme production. Applications of cellulases and xylanases produced by this fungus are found in food, animal feed, pharmaceutical, textile and pulp and paper industries. T. reesei is non-pathogenic for man and it has been shown not to produce fungal toxins or antibiotics under ...
H, Nevalainen, P, Suominen, K, Taimisto
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Molecular Identification of Trichoderma reesei
2020Fungi comprise one of the most diverse groups of eukaryotes with many cryptic species that are difficult to identify. In this chapter, we detail a protocol for the molecular identification of the most industrially relevant species of Trichoderma-T. reesei.
Mohammad J, Rahimi +4 more
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Ecological Genomics and Evolution of Trichoderma reesei
2020The filamentous fungus Trichoderma reesei (Hypocreales, Ascomycota) is an efficient industrial cell factory for the production of cellulolytic enzymes used for biofuel and other applications. Therefore, researches addressing T. reesei are relatively advanced compared to other Trichoderma spp.
Komal, Chenthamara +4 more
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Proteomic Profiling of the Secretome of Trichoderma reesei
2020Trichoderma reesei (T. reesei) is the workhorse for the production of industrial cellulolytic enzyme cocktails for cellulose hydrolysis. However, the current industrial process using enzyme cocktails is not efficient enough for the cost-effective generation of cellulosic sugar.
So Fong Cam, Ngan, Siu Kwan, Sze
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Characterization of the Trichoderma reesei cbh2 promoter
Current Genetics, 1993A 613-bp fragment of the 5' upstream region of the Trichoderma reesei cbh2 gene (coding for the cellulolytic enzyme cellobiohydrolase II) has been isolated and sequenced. Fusion of this fragment to the E. coli uidA gene (coding for beta-glucuronidase) leads to--albeit low--expression of beta-glucuronidase activity in the presence of cellulose and upon ...
H, Stangl, F, Gruber, C P, Kubicek
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The Potential of Synthetic Biology for Trichoderma reesei
2020Within the last 20 years, ground-breaking progress has been made in the field of synthetic biology, enabling the construction of novel pathways up to entire synthetic genomes in both prokaryotic and eukaryotic organisms. These innovations are primarily adapted for biotechnological applications, where filamentous fungi such as Trichoderma reesei are ...
Roland, Martzy, Astrid R, Mach-Aigner
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Batch Cultivation of Trichoderma reesei
2020This chapter explains how to perform a batch cultivation of Trichoderma reesei in bench top bioreactors, exemplarily using wheat straw as sole carbon source, and a selection of recommended, frequently used analyses to monitor the cultivation (intra- and extracellular as well), which are microscopic analysis, sodium hydroxide soluble protein, Bradford ...
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Sphaeroplast formation and regeneration in Trichoderma reesei
European Journal of Applied Microbiology and Biotechnology, 1981Sphaeroplasts from several genetically marked strains of Trichoderma reesei were readily prepared through enzymatic hydrolysis of mycelial suspensions utilizing Driselase, a commercially available lytic enzyme preparation. Sphaeroplasts were released from the apical tips of hyphae after 90 min exposure to the enzyme and, with longer treatments, from ...
S. K. Picataggio +3 more
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RNA Characterization in Trichoderma reesei
2020This chapter provides an overview on different methods for the characterization of RNAs in Trichoderma reesei. In the first section, protocols for the extraction of total RNA from fungal mycelia and the identification of 5' and 3' ends of certain RNAs of interest via rapid amplification of cDNA ends (RACE) are presented.
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