Results 121 to 130 of about 111,207 (280)

Padronização da metodologia do RT-PCR utilizado para identificação do mRNA da a-amilase em sementes de milho. [PDF]

, 2019
Objetivou-se padronizar a metodologia do RT-PCR paraidentificar o mRNA do gene de ?-amilase em sementes de milho. Após três dias de germinaçãodas cultivares Saracura-BRS 4154 e CATI-AL34, extraiu-se o RNA total pelo método do tiocianatode guanidina-fenol-
ARAGÃO, C. A., ARAÚJO JÚNIOR, J. P., CAVARIANI, C., DANTAS, B. F., NAKAGAWA, J., RODRIGUES, J. D.   +5 more
core  

Comparison of Fecal MicroRNA Isolation Using Various Total RNA Isolation Kits

Genes
Fecal specimens have long been regarded as promising sources for gastrointestinal cancer screening and have, thus, been extensively investigated in biomarker research.
Theresa Lederer, N. M. Hipler, Cosima Thon, J. Kupčinskas, Alexander Link   +4 more
semanticscholar   +1 more source

Cell Surface Thiol Engineering Mechanoregulates Myogenic Differentiation via the FAK–PI3K–AKT Axis

Advanced Healthcare Materials, EarlyView.
Schematic diagram illustrating how cell surface modification of skeletal muscle progenitor cells through TCEP treatment reveals enhanced cell adhesion, intracellular tension, and myogenesis at 19.66 kPa stiffness, leading to optimal cell fusion. In contrast, no significant changes are observed in the softer (10.61 kPa) or stiffer (49.4 kPa) matrices ...
Juyeon Kim, Sung Sik Hur, Jae Hong Park, Myung Jin Ban, Jin‐Young Kim, Joo Hyun Kim, Ji Hyae Choo, Hae‐Won Kim, Ju Hun Lee, Man Ryul Lee, Yongsung Hwang   +10 more
wiley   +1 more source

Tumor necrosis factor-alpha regulates the expression of inducible costimulator receptor ligand on CD34+ progenitor cells during differentiation into antigen presenting cells [PDF]

, 2001
The inducible costimulator receptor (ICOS) is a third member of the CD28 receptor family that regulates T cell activation and function. ICOS binds to a newly identified ligand on antigen presenting cells different from the CD152 ligands CD80 and CD86. We
Aicher, A, Clark, EA, Daemen, K, Hayden-Ledbetter, M, Irgang, M, Korner, I, Ledbetter, JA, Pezzutto, A, Richter, G, Westermann, J   +9 more
core  

An Organ‐on‐Chip Platform for Strain‐Controlled, Tissue‐Specific Compression of Cartilage and Mineralized Osteochondral Interface to Study Mechanical Overloading in Osteoarthritis

Advanced Healthcare Materials, EarlyView.
A mechanically active OsteoChondral Unit (OCU)‐on‐Chip platform mimicking the OCU's functional anatomy and the strain gradient across the osteochondral interface is presented. Upon compartment‐specific hyperphysiological compression, the model replicates mechanisms observed in osteoarthritis (OA) progression, such as calcium crystal accumulation ...
Andrea Mainardi, Anastasiya Börsch, Paola Occhetta, Robert Ivanek, Martin Ehrbar, Lisa Krattiger, Philipp Oertle, Marko Loparic, Ivan Martin, Marco Rasponi, Andrea Barbero   +10 more
wiley   +1 more source

THE APLICATION OF REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF CANINE DISTEMPER

Jurnal Veteriner, 2008
A study was conducted to apply reverse transcriptase-polymerase chain reaction (RT-PCR) technique for the confirmative diagnosis of canine distemper in dogs. Twenty mongreal dogs with clinical symptoms of canine distemper were used in this study.
I Nyoman Suartha, I Gusti Ngurah Kade Mahardika, Ida Ayu Sri Candra Dewi, Ni Ketut Dias Nursanty, Yosaphat L.S Kote, Anita Dwi Handayani, I Gusti Agung Ayu Suartini   +6 more
doaj  

Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal

BMC Biotechnology, 2018
BackgroundmicroRNAs (miRNAs) are short non-coding RNAs that fine-tune gene expression. The aberrant expression of miRNAs is associated with many diseases and they have both therapeutic and biomarker potential.
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, T. D. Kabir, Kirsty L. Richardson, P. Leedman   +5 more
semanticscholar   +1 more source

DNA Barcoding‐Enabled Tracking of Lipid Nanoparticles: Drug‐Loading‐Dependent Biodistribution and Tumor Microenvironment Targeting

Advanced Healthcare Materials, EarlyView.
In this study, lipid nanoparticles are tracked in vivo using DNA barcodes to determine how varying drug loadings affect their delivery behaviors in mice. A cost‐effective strategy is developed for simultaneously monitoring the biodistribution of a library of lipid nanoparticles (LNPs).
Letao Xu, Rui Chen, Xing Wang, Dawei Liu, Yun liu, Chun‐Xia Zhao   +5 more
wiley   +1 more source

A cost-effective RNA extraction and RT-qPCR approach to detect California serogroup viruses from pooled mosquito samples

Scientific Reports
Mosquito-borne diseases pose ongoing global health concerns, demanding more cost-efficient methods to detect pathogens to support enhanced surveillance efforts.
Marc Avramov, Vanessa Gallo, Antonia Gross, D. Lapen, Antoinette Ludwig, Catherine I. Cullingham   +5 more
semanticscholar   +1 more source

3D Bioprinting‐Assisted Engineering of Stem Cell‐Laden Hybrid Biopatches With Distinct Geometric Patterns Considering the Mechanical Characteristics of Regular and Irregular Connective Tissues

Advanced Healthcare Materials, EarlyView.
A hybrid biopatch platform integrating 3D printed polymeric patterns with stem cell‐laden collagen bioinks is developed to mimic the mechanical properties of connective tissues. By tailoring geometric architectures, the constructs replicate anisotropic or isotropic mechanics, enhancing tissue‐specific regeneration.
Minjun Ahn, Gyu Tae Park, Arvind Kumar Shukla, Boguen Kwon, Jae Ho Kim, Eui‐Suk Sung, Byoung Soo Kim   +6 more
wiley   +1 more source