Results 131 to 140 of about 111,207 (280)

Adapted protocol to extract total RNA using TRIzol® (Invitrogen, Thermo) v1 [PDF]

, 2019
Talita Bianca Gagliardi
openalex   +1 more source

Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis

Advanced Healthcare Materials, EarlyView.
Crypt‐villi‐like morphogenesis can be driven and controlled via architectural patterns in colorectal cells with the use of digital light processing printing. The resulting mini tissues exhibit budding structures and functionality, as evidenced by increased mucin expression.
Nathaniel C. Burmas, Arlyn M. Fabian, Amoli Vanavadiya, Quinton Smith   +3 more
wiley   +1 more source

Comprehensive Assessment of Inactivation Methods for Madariaga Virus

Viruses
The Eastern Equine Encephalitis Virus (EEEV) is an emerging public health threat, with the number of reported cases in the US increasing in recent years. EEEV is a BSL3 pathogen, and the North American strain is a US Federal Select Agent (SA).
RuthMabel Boytz, Kadiatou Keita, Joanna B. Pawlak, Maudry Laurent-Rolle   +3 more
semanticscholar   +1 more source

Biomimetic Scaffolds with Dual Gradients of Biological Effectors for Tendon‐to‐Bone Repair

Advanced Healthcare Materials, EarlyView.
The authors have developed a biomimetic scaffold with dual gradients through a diffusion‐driven method. The scaffold features funnel‐shaped microchannels for cell infiltration, while the dual gradients synergetically induce the graded differentiation of mesenchymal stem cells into osteoblasts, chondrocytes, and tenocytes, allowing for a seamless ...
Min Hao, Yidan Chen, Stavros Thomopoulos, Younan Xia   +3 more
wiley   +1 more source

Paracoccidioides spp.: the structural characterization of extracellular matrix, expression of glucan synthesis and associated genes and adhesins during biofilm formation

Frontiers in Microbiology
The genus Paracoccidioides includes Paracoccidioides lutzii and the Paracoccidioides brasiliensis complex, which comprises four phylogenetic species. A key feature distinguishing planktonic growth from biofilm is the presence of a 3D extracellular matrix
Lariane Teodoro Oliveira, Caroline Maria Marcos, Ana Karla Lima Freire Cabral, Ana Karla Lima Freire Cabral, Kaila Petronila Medina-Alarcón, Regina Helena Pires, Ana Marisa Fusco-Almeida, Maria José Soares Mendes-Giannini   +7 more
doaj   +1 more source

Evaluation and Standardization of RNA Extractions with Quality for RNA-Seq for Balamuthia mandrillaris

Parasitologia
Balamuthia mandrillaris is a free-living amoeba (FLA) that causes granulomatous amebic encephalitis (GAE) and skin lesions. Transcriptomic analysis is a powerful tool used to study B. mandrillaris pathogenic infections.
Leobardo Daniel Gonzalez-Zuñiga, L. Z. Rodriguez-Anaya, J. R. Gonzalez-Galaviz, Abraham Cruz-Mendívil, F. Lares-Villa, L. F. Lares-Jiménez   +5 more
semanticscholar   +1 more source

Development of a Modular Ribonucleoprotein Complex as a General Strategy to Deliver RNAi Therapeutics

Advanced Healthcare Materials, EarlyView.
A modular and customizable RNAi therapeutics delivery platform composed of a pseudosymmetric human RNA binding protein‐siRNA complex (SmiRNP) is developed. By fusing a targeting module and an endosomal escape peptide onto the delivery platform, SmiRNP successfully delivered siRNA to silence the KRAS oncogene in a colorectal carcinoma cell line, leading
Nok Yin Tam, Xiaoqi Wang, Grace Chung Yan Chan, Wai Po Kong, Wai Yin Chau, Xiuqiong Fu, Kwok‐Yin Wong, Hong Lok Lung, Zhi‐Ling Yu, Wei Shen Aik   +9 more
wiley   +1 more source

Nanoinjection Platform for Drug Delivery in Breast Cancer Therapeutics

Advanced Materials Interfaces, EarlyView.
A schematic shows Nanoarchaeosomes loaded with doxorubicin (NAD) delivered via silicon nanotubes (SiNTs) into MCF‐7 cells, triggering apoptosis and necrosis. Released doxorubicin generates ROS, disrupts mitochondria, and intercalates DNA. This dual‐action nanoinjection enhances cytotoxicity and improves breast cancer therapy efficacy.
Kaviya Vijayalakshmi Babunagappan, Subastri Ariraman, Jann Harberts, Vimalraj Selvaraj, Mukilarasi Bedatham, Narendran Sekar, Nicolas H Voelcker, Roey Elnathan, Swathi Sudhakar   +8 more
wiley   +1 more source

4D Bioprinted Self‐Folding Scaffolds Enhance Cartilage Formation in the Engineering of Trachea

Advanced Materials Technologies, Volume 10, Issue 6, March 18, 2025.
A bilayer self‐folding scaffold, triggerable by humidity, is fabricated via 4D bioprinting for trachea engineering. An analytical model is derived to predict its radius of curvature, enabling its scalability. Cartilage progenitor cells seeded on the scaffold perceive scaffold final curvature and react to it, by enhancing the upregulation of pro ...
Irene Chiesa, Alessio Esposito, Giovanni Vozzi, Riccardo Gottardi, Carmelo De Maria   +4 more
wiley   +1 more source

SMYD3 Promotes Immune Evasion in Clear Cell Renal Cell Carcinoma via SREBP1‐Mediated Transactivation of CD47

Advanced Science, EarlyView.
Cancer cell‐intrinsic SMYD3 orchestrates an immunosuppressive microenvironment and impairs the response to PD‐1 blockade by reprogramming immune cells landscape in the tumor microenvironment of clear cell renal cell carcinoma (ccRCC). The immunomodulatory function of SMYD3 is mediated via the SREBP1/CD47 pathway.
Zhengfang Liu, Xiumei Zhao, Maolin Zang, Huiyang Yuan, Xin Qin, Xiaofeng Li, Shuo Zhao, Ruirong Tan, Keqiang Yan, Li Liu, Yidong Fan, Ning Zhang, Benkang Shi, Bo Han, Shouzhen Chen   +14 more
wiley   +1 more source