Results 61 to 70 of about 111,207 (280)
Int. J. Comput. Biol. Drug Des., 2014 MicroRNAseq (miRNAseq) is a form of RNAseq technology that has become an increasingly popular alternative to miRNA expression profiling. Unlike messenger RNA (mRNA), miRNA extraction can be difficult, and sequencing such small RNA can also be problematic.
Yan Guo +5 more
semanticscholar +1 more source
Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2023 Increasing evidence of sperm RNA's role in fertilization and embryonic development has provided impetus for its isolation and thorough characterization. Sperm are considered tough-to-lyse cells, due to compact condensed DNA in sperm heads.
Saurabh Tiwari +4 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.Microglia (BV2) and IL‐4‐treated BMDMs promote astrocytoma clustering and inhibit tumor growth, not shown in other macrophage cells. In vivo, microglial co‐implantation enhances CD8+ T cell infiltration, elevates Granzyme B, lowers circulating MDSCs, and extends survival only in immune‐competent mice, but not in immune‐deficient mice.
Tzu‐Chieh Sun +5 more
wiley +1 more source
Insight of PBZ mediated drought amelioration in crop plants
Frontiers in Plant Science, 2022 Water scarcity is a significant environmental limitation to plant productivity as drought-induced crop output losses are likely to outnumber losses from all other factors.
Chirag Maheshwari +6 more
semanticscholar +1 more source
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Malignant gliomas pose significant therapeutic challenges. This study aimed to identify and characterize a novel chimeric RNA in glioma and assess its clinical and functional significance for precision treatment. Methods The C19orf47‐AKT2 chimeric RNAs were identified through RNA sequencing and validated by polymerase chain reaction.
Zihan Wang +11 more
wiley +1 more source
Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma
Microbial Cell Factories, 2020 Background Inclusion bodies (IBs) are protein aggregates in recombinant bacterial cells containing mainly the target recombinant protein. Although it has been shown that IBs contain functional proteins along with protein aggregates, their direct ...
Elena Krachmarova +2 more
doaj +1 more source
Incubation temperature affects type X collagen expression in embryonic ducks [PDF]
, 2014 Optimal temperature regulation is an important component of avian incubation and the process of embryonic development can be accelerated by even small increases in incubation temperature.
Prickett, Amanda L.
core
Standard methods for molecular research in Apis mellifera [PDF]
, 2013 From studies of behaviour, chemical communication, genomics and developmental biology, among many others, honey bees have long been a key organism for fundamental breakthroughs in biology.
Budge, G. +19 more
core +1 more source
Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays
Journal of Infectious Diseases, 2020 The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease.
Edward I. Patterson +10 more
semanticscholar +1 more source
Advanced Biology, EarlyView.The axolotl's remarkable regenerative abilities decline with age, the causes may include the numerous repetitive elements within its genome. This study uncovers how Ty3 retrotransposons and coexpression networks involving muscle and immune pathways respond to aging and regeneration, suggesting that transposons respond to physiological shifts and may ...
Samuel Ruiz‐Pérez +8 more
wiley +1 more source