Optimized microRNA purification from TRIzol-treated plasma
BMC Genomics, 2015 BackgroundMicroRNAs (miRNAs) represent new and potentially informative diagnostic targets for disease detection and prognosis. However, little work exists documenting the effect of TRIzol, a common viral inactivation and nucleic acid extraction reagent ...
J. Duy, J. Koehler, A. Honko, T. Minogue
semanticscholar +1 more source
Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays
Journal of Infectious Diseases, 2020 The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease.
Edward I. Patterson +10 more
semanticscholar +1 more source
Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma
Molecular Oncology, EarlyView.Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken +7 more
wiley +1 more source
The Acute Responses of Different-sized Coronary Arteries to Testosterone [PDF]
, 2012 Coronary arteries supply blood to the myocardium. The blood flow within the coronary arteries is altered by various compounds produced within the body. Sex hormones such as testosterone are known to cause the relaxation of large coronary arteries.
Madanu, Sampath
core +2 more sources
Small RNA sequencing of cryopreserved semen from single bull revealed altered miRNAs and piRNAs expression between High- and Low-motile sperm populations [PDF]
, 2017 Putative piRNA clusters that were assigned by proTRAC to the High Motile (HM) and Low Motile (LM) sperm fractions.
A. Stella +7 more
core +9 more sources
TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton
Scientific Reports, 2015 Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton ...
J. Preston Campbell +8 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.Meta‐transcriptome analysis identified FGF19 as a peptide enteroendocrine hormone associated with colorectal cancer prognosis. In vivo xenograft models showed release of FGF19 into the blood at levels that correlated with tumor volumes. Tumoral‐FGF19 altered murine liver metabolism through FGFR4, thereby reducing bile acid synthesis and increasing ...
Jordan M. Beardsley +5 more
wiley +1 more source
Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma
Microbial Cell Factories, 2020 Background Inclusion bodies (IBs) are protein aggregates in recombinant bacterial cells containing mainly the target recombinant protein. Although it has been shown that IBs contain functional proteins along with protein aggregates, their direct ...
Elena Krachmarova +2 more
doaj +1 more source
Macrophage migration inhibitory factor (MIF) family in arthropods : Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in Mud crabs, Scylla paramamosain [PDF]
, 2016 Acknowledgements This research was supported by grants from the National Natural Science Foundation of China (Nos. 31172438 and U1205123), the Natural Science Foundation of Fujian Province (No.
Duan, Li-Peng +7 more
core +1 more source
Molecular Oncology, EarlyView.Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova +25 more
wiley +1 more source