Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples
Brazilian Journal of Infectious DiseasesThe efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil.
Vanessa Salete de Paula +2 more
doaj +1 more source
High-quality RNA extraction from the sea urchin Paracentrotus lividus embryos.
PLoS ONE, 2017 The sea urchin Paracentrotus lividus (Lamarck, 1816) is a keystone herbivore in the Mediterranean Sea due to its ability to transform macroalgal-dominated communities into barren areas characterized by increased cover of bare substrates and encrusting ...
Nadia Ruocco +6 more
doaj +1 more source
Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma
Microbial Cell Factories, 2020 Background Inclusion bodies (IBs) are protein aggregates in recombinant bacterial cells containing mainly the target recombinant protein. Although it has been shown that IBs contain functional proteins along with protein aggregates, their direct ...
Elena Krachmarova +2 more
doaj +1 more source
Effect of Long-term Storage in TRIzol on Microarray-Based Gene Expression Profiling
, 2010 Background: Although TRIzol is widely used for preservation and isolation of RNA, there is suspicion that prolonged sample storage in TRIzol may affect array-based gene expression profiling (GEP) through premature termination during reverse transcription.
Larry W. Kwak +14 more
core +1 more source
TRIzol-based RNA extraction for detection protocol for SARS-CoV-2 of coronavirus disease 2019
, 2021 International audienceDiagnostic testing is important for managing the 2019 novel coronavirus (SARS-CoV-2). We developed an optimized protocol for SARS-CoV-2 RNA extraction from the surface of the respiratory mucosa with nasopharyngeal swabs and compared
Amirouche, A. +6 more
core +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
PLoS ONE, 2019 Gene expression profiling using blood samples is a valuable tool for biomarker discovery in clinical studies. Different whole blood RNA collection and processing methods are highly variable and might confound comparisons of results across studies.
Aarti Gautam +10 more
doaj +1 more source
A simple and efficient method for isolating small RNAs from different plant species
Plant Methods, 2011 Background Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging
de Folter Stefan +5 more
doaj +1 more source
RNA Extraction from Drosophila Tissues using TRIzol Reagent v1
, 2016 This protocol is adapted from the Invitrogen Life Technologies Trizol manual.
Elizabeth Allen
core +1 more source
BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells
FEBS Open Bio, EarlyView.BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley +1 more source