Results 41 to 50 of about 616,340 (230)

Diagnostic value of Xpert® BC Detection, Bladder Epicheck®, Urovysion® FISH and cytology in the detection of upper urinary tract urothelial carcinoma. [PDF]

, 2023
PURPOSE Following the current guidelines, diagnosis and staging for upper urinary tract tumours (UTUC) can be performed with Computed Tomography, urography, ureterorenoscopy (URS) and selective cytology.
Danuser, Hansjörg, Pycha, Armin, Christine Schwienbacher, Hansjörg Danuser, Carolina D’Elia, Hanspeter, Esther, Mian, Christine, D'Elia, Carolina, Esther Hanspeter, Margherita Palermo, Stefan Pycha, Emanuela Trenti, Trenti, Emanuela, Armin Pycha, Schwienbacher, Christine, Pycha, Stefan, Palermo, Margherita, Christine Mian   +17 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

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, 2014
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core   +1 more source

Optimized TRIzol extraction of DNA from archived specimens.

, 2012
(A) Schematic representation of DNA recovery from the lower phase of TRIzol (upper phase yields RNA). In step 1 (yellow bullet), tissue digestion is performed following the procedure described in Loudig et al. 2007.
Kenny Ye (79112), Susan Fineberg (171525), Ryung Kim (171500), Andrew Ramnauth (171480), Juan Lin (171491), Thomas Rohan (171514), Olivier Loudig (171530), Adam Kotorashvili (171474), Rachel Hazan (171509), Christina Liu (171485)   +9 more
core   +1 more source

Inhalation characteristics of asthma patients, COPD patients and healthy volunteers with the Spiromax® and Turbuhaler® devices: a randomised, cross-over study. [PDF]

, 2015
BACKGROUND: Spiromax® is a novel dry-powder inhaler containing formulations of budesonide plus formoterol (BF). The device is intended to provide dose equivalence with enhanced user-friendliness compared to BF Turbuhaler® in asthma and chronic ...
Philip Chetcuti, Hosker, Harold, Chrystyn, Henry, Azouz, Wahida, Saralaya, Dinesh, Dinesh Saralaya, Chetcuti, Philip, Wahida Azouz, Henry Chrystyn, Harold Hosker   +9 more
core   +2 more sources

Post-TRIzol protein extraction from peripheral blood mononuclear cells [PDF]

, 2023
After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to the limited efficiency of pellet reconstitution/solubilisation. As the pelleted proteins are tightly packed, they are most often solubilised using
Stevanović Jovana, Robajac Dragana, Nedić Olgica, Dobrijević Zorana   +3 more
core   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Isolation of RNA from adipose tissue using TRIzol reagent

, 2021
The ability to isolate quality RNA from a sample is an integral component for studying gene expression. By studying transcribed RNA, researchers can observe changes in gene expression and investigate the role of specific genes that are expressed in ...
Lukwesa, Mukobe
core   +1 more source

Proceedings for RNA extraction from cell cultures using phenol-chloroform method with TRIzol ® (Ambion) reagent v1

, 2018
Goal This document has the objective of establishing a proceeding for RNA extraction from eukaryotic cells through phenol-chloroform method using TRIzol (Ambion) reagent.
Maria Cristina Vidal Pessolani, João Pedro Santos   +1 more
core   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source