Applied BiosciencesDeformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread ...
Krisztina Christmon +3 more
doaj +1 more source
The atypical chemokine receptor ACKR2 is protective against sepsis [PDF]
, 2018 Sepsis is a systemic inflammatory response as a result of uncontrolled infections. Neutrophils are the first cells to reach the primary sites of infection and chemokines play a key role in recruiting neutrophils.
Alves-Filho, José Carlos +15 more
core +1 more source
Journal of Limnology, 2007 A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN +3 more
doaj +1 more source
Comparison of chemical profiles and effectiveness between Erxian decoction and mixtures of decoctions of its individual herbs : a novel approach for identification of the standard chemicals [PDF]
, 2017 Acknowledgements This study was partially supported by grants from the Seed Funding Programme for Basic Research (Project Number 201211159146 and 201411159213), the University of Hong Kong.
Cheung, H. P. +8 more
core +1 more source
Genome-Wide Association Study and Subsequent Exclusion of ATCAY as a Candidate Gene Involved in Equine Neuroaxonal Dystrophy Using Two Animal Models. [PDF]
, 2020 Equine neuroaxonal dystrophy/equine degenerative myeloencephalopathy (eNAD/EDM) is an inherited neurodegenerative disorder of unknown etiology. Clinical signs of neurological deficits develop within the first year of life in vitamin E (vitE) deficient ...
Dahlgren, Anna R +6 more
core +1 more source
Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™
BioTechniques, 2018 The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents.
doaj +1 more source
Avaliação de diferentes métodos para extração de RNA total de folhas e raízes de braquiária. [PDF]
, 2012 A extração de RNA a partir de tecidos específicos consiste no primeiro passo para estudos de expressão gênica e caracterização de transcritos. Plantas, em geral, contêm grande quantidade de compostos fenólicos e/ou polissacarídeos em seus tecidos, o que ...
BITENCOURT, G. de A. +4 more
core
Distribution and viability of fetal and adult human bone marrow stromal cells in a biaxial rotating vessel bioreactor after seeding on polymeric 3D additive manufactured scaffolds [PDF]
, 2015 One of the conventional approaches in tissue engineering is the use of scaffolds in combination with cells to obtain mechanically stable tissue constructs in vitro prior to implantation.
Blitterswijk, Clemens A. van +3 more
core +3 more sources
Molecular Oncology, EarlyView.Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova +25 more
wiley +1 more source
Phenolic metabolites of anthocyanins modulate mechanisms of endothelial function [PDF]
, 2015 Anthocyanins are reported to have vascular bioactivity, however their mechanisms of action are largely unknown. Evidence suggests that anthocyanins modulate endothelial function, potentially by increasing nitric oxide (NO) synthesis, or enhancing NO ...
Cassidy, Aedin +4 more
core +1 more source