Results 21 to 30 of about 616,340 (230)

Comparación de métodos de extracción de RNA para la detección por RT-PCR del Potato yellow vein virus (PYVV) en diferentes órganos de Solanum tuberosum Grupo Phureja

Revista Colombiana de Biotecnología, 2013
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán, Mónica Guzmán-Barney   +1 more
doaj   +2 more sources

Gene expression profiling of whole blood: A comparative assessment of RNA-stabilizing collection methods.

PLoS ONE, 2019
Peripheral Blood gene expression is widely used in the discovery of biomarkers and development of therapeutics. Recently, a spate of commercial blood collection and preservation systems have been introduced with proprietary variations that may ...
Duncan E Donohue, Aarti Gautam, Stacy-Ann Miller, Seshamalini Srinivasan, Duna Abu-Amara, Ross Campbell, Charles R Marmar, Rasha Hammamieh, Marti Jett   +8 more
doaj   +1 more source

Canine distemper virus detection in asymptomatic and non vaccinated dogs

Pesquisa Veterinária Brasileira, 2010
A quantitative real time polymerase chain reaction (PCR) revealed canine distemper virus presence in peripheral blood samples from asymptomatic and non vaccinated dogs.
Helen L. Del Puerto, Anilton C. Vasconcelos, Luciana Moro, Fabiana Alves, Gissandra F. Braz, Almir S. Martins   +5 more
doaj   +1 more source

Modulation of atrazine-induced chromosomal aberrations and cyclin-dependent kinases by aqueous extract of Roylea cinerea (D.Don) Baillon leaves in Allium cepa

Scientific Reports, 2022
Roylea cinerea (D.Don) Baillon an indigenous medicinal plant of Lamiaceae family used for the treatment of several diseases. In the present study, its aqueous (leaves) extract was tested for genoprotective action against atrazine-induced chromosomal ...
Farhana Rashid, Davinder Singh, Shivani Attri, Prabhjot Kaur, Harneetpal Kaur, Pallvi Mohana, Jahangeer Quadar, Adarsh Pal Vig, Astha Bhatia, Balbir Singh, Harpreet Walia, Saroj Arora   +11 more
doaj   +1 more source

Quantification and qualification of Trizol-DNA.

, 2016
(A) Trizol-DNA was serially diluted with distilled water, and the concentration of each diluent was measured by NanoDrop (circles), BR-Qubit (squares), HS-Qubit (diamonds) and qPCR (triangles). The broken line indicates the expected NanoDrop value.
Naoki Einaga (2551768), Mariko Esumi (2551771), Hiromi Yamaguchi (2396248), Yuki Nakayama (365318)   +3 more
core   +1 more source

Immunocapture RT-qPCR Method for DWV-A Surveillance: Eliminating Hazardous Extraction for Screening Applications

Applied Biosciences
Deformed wing virus (DWV) is a major contributor to honey bee colony losses, making effective monitoring essential for apiary management. Traditional DWV detection relies on hazardous RNA extraction followed by RT-qPCR, creating barriers for widespread ...
Krisztina Christmon, Eugene V. Ryabov, James Tauber, Jay D. Evans   +3 more
doaj   +1 more source

Simple Modifications to Standard TRIzol® Protocol Allow High-Yield RNA Extraction from Cells on Resorbable Materials

, 2011
Resorbable bioceramics are attractive for medical applications such as bone substitution. Biochemical analysis on cells cultured on these biomaterials is vital to predict the impact of the materials in vivo and RNA extraction is an essential step in gene
Juliana Tsz Yan Lee, Tsang, Wai Hung, Wai Hung Tsang, King Lau Chow, Chow, King Lau, Lee, Juliana Tsz Yan   +5 more
core   +1 more source

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

Journal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN, Jochen D'HAESE, Hieronymus DASTYCH, Ernst KIEHL   +3 more
doaj   +1 more source

RNA isolation with TRIzol v1

, 2018
Standard RNA isolation protocol using TRIzol
Magdalena Julkowska
core   +1 more source

Collecting, archiving and processing DNA from wildlife samples using FTA® databasing paper [PDF]

, 2004
Background Methods involving the analysis of nucleic acids have become widespread in the fields of traditional biology and ecology, however the storage and transport of samples collected in the field to the laboratory in such a manner to allow ...
LA Burgoyne, Smith, L M, LM Smith, Burgoyne, Leigh Alexander   +3 more
core   +1 more source