Results 1 to 10 of about 616,340 (230)
High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
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COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
Revista Brasileira de Fruticultura, 2016 Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ +2 more
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BioTechniques, 2007 A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic ...
Amanda B. Hummon +3 more
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An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]
Bio-ProtocolOsteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj +2 more
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LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]
Journal of Cell and Molecular Research, 2021 Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi +2 more
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Frontiers in Agronomy, 2022 Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang +32 more
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Protocol for quantifying miRNA trafficking across the endosomal membrane. [PDF]
FEBS Open BioAn in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Ghosh S, Mukherjee K, Bhattacharyya SN.
europepmc +2 more sources
Rodriguésia, 2020 Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha +4 more
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First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia [PDF]
Veterinary World, 2021 Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the
Héctor Contreras +5 more
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Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology
Acta Stomatologica Croatica, 2016 Objective of work: The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real ...
Mônica Ghislaine Oliveira Alves +9 more
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