Comparison of different methods for preparation and characterization of total RNA from cartilage samples to uncover osteoarthritis
BMC Research Notes, 2010 Background The isolation of intact RNA can be very difficult when tissues are used that contain many RNAses or that are hard to homogenize, e.g. cartilage samples.
Neumann Steffi +3 more
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An Optimized RNA Extraction Method From Micro-quantities of Guinea Pig Cartilage and Synovium for Osteoarthritis Research [PDF]
Bio-ProtocolOsteoarthritis (OA) is the primary cause of joint impairment, particularly in the knee. The prevalence of OA has significantly increased, with knee OA being a major contributor whose pathogenesis remains unknown. Articular cartilage and the synovium play
Nidhi Bhardwaj +2 more
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Peripheral Blood IL-6 and STAT3 Gene Expression as Biomarkers Associated with Neoadjuvant Chemotherapy Response in Triple-Negative Breast Cancer: A Prospective Cross-Sectional Study [PDF]
Breast Cancer: Targets and TherapyKayhan Özdemir,1 Ali Muhtaroğlu,2 Muhammet Burak Kamburoğlu,3 Gamze Guney Eskiler,4 Zeynep Özman Gökçe,5 Belma Kocer6 1Department of General Surgery, Sakarya University Faculty of Medicine, Sakarya, Turkey; 2Department of General ...
Özdemir K +5 more
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LncRNA HOXD-AS1 Is Upregulated in Ovarian Cancer [PDF]
Journal of Cell and Molecular Research, 2021 Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis.
Seyedeh Nahid Fotuhi +2 more
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Frontiers in Agronomy, 2022 Citrus germplasm programs can benefit from high-throughput polymerase chain reaction (PCR)-based methods for the detection of graft-transmissible pathogens in propagative materials.
Tyler Dang +32 more
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A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia [PDF]
, 2020 © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Arenas Gomez, C. M., Woodcock, M. R., Smith, J. J., Voss, S. R., & Delgado, J. P. A de novo reference
Arenas Gómez, Claudia Marcela +4 more
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Rodriguésia, 2020 Considering the lack of information on RNA extraction from arboreal species, specially from the Brazilian Cerrado, the aim of this study was to test RNA extraction methods for a wide variety of native plant species from this biome.
Geisiane Alves Rocha +4 more
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High-yield skeletal muscle protein recovery from TRIzol after RNA and DNA extraction
BioTechniques, 2020 Extraction of DNA, RNA and protein from the same sample would allow for direct comparison of genomic, transcriptomic and proteomic information. Commercially available kits exhibit poor protein yield and the TRIzol® reagent produces a protein pellet that ...
Yuan Wen +3 more
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First report of tilapia lake virus emergence in fish farms in the department of Córdoba, Colombia [PDF]
Veterinary World, 2021 Background and Aim: In 2016, the tilapia-producing farms in the department of Córdoba, Colombia, had witnessed outbreaks of disease with clinical signs compatible with those caused by the tilapia lake virus (TiLV). This study was conducted to confirm the
Héctor Contreras +5 more
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COMPARISON OF RNA EXTRACTION METHODS FOR Passiflora edulis SIMS LEAVES
Revista Brasileira de Fruticultura, 2016 Functional genomic analyses require intact RNA; however, Passiflora edulis leaves are rich in secondary metabolites that interfere with RNA extraction primarily by promoting oxidative processes and by precipitating with nucleic acids. This study aimed to
ANNY CAROLYNE DA LUZ +2 more
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