Results 11 to 20 of about 616,340 (230)

Alkaline water modules the expression of inflammation genes: in vitro model for cancer prevention [PDF]

open access: yesWorld Cancer Research Journal, 2020
OBJECTIVE: Many researches focus their attention on the behavior of cells to specific substances with alkalizing power. Each substance that we introduce with food, as well as water, contributes to conditioning the electrophilic balance (acid-base) of the
A. Del Buono   +5 more
doaj   +1 more source

A modified CTAB and Trizol® protocol for high-quality RNA extraction from whole wheat seedlings, including rhizosphere

open access: yes, 2020
The RNA extraction was performed from foliar (F) and whole wheat plants (including rhizosphere) (WP) samples by (1) the standard TRIzol® protocol, and (2) a modifed CTAB and TRIzol® protocol.
de los Santos-Villalobos, S.   +3 more
core   +1 more source

pH-sensing G protein-coupled orphan receptor GPR68 is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progression [PDF]

open access: yesPeerJ, 2023
Background Osteoarthritis (OA) is a debilitating joints disease affecting millions of people worldwide. As OA progresses, chondrocytes experience heightened catabolic activity, often accompanied by alterations in the extracellular environment’s ...
Nazir M. Khan   +5 more
doaj   +2 more sources

Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

open access: yesBiomedicines, 2021
MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis.
Luis Javier Martínez-González   +8 more
doaj   +1 more source

Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell Cultures

open access: yesMethods and Protocols, 2021
Given that the airway epithelium is the initial site of infection, study of primary human airway epithelial cells (AEC) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) will be crucial to improved understanding of viral entry ...
Kaitlyn A. Barrow   +6 more
doaj   +1 more source

Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens

open access: yesMemorias do Instituto Oswaldo Cruz, 2010
This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies.
Cleiton Paulo Aigner   +5 more
doaj   +1 more source

Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis

open access: yesMethodsX, 2019
Dirofilaria immitis, the etiologic agent of canine heartworm disease, like several other filarial nematodes, harbors the bacterial endosymbiont Wolbachia. To investigate metabolic and functional pathways of D.
Chiara Lucchetti   +5 more
doaj   +1 more source

Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid

open access: yesPlants, 2013
Jerusalem artichoke (Helianthus tuberosus L.) is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis ...
Tanupat Mornkham   +5 more
doaj   +1 more source

GENES DE REFERÊNCIA PARA ESTUDOS DE EXPRESSÃO RELATIVA POR QPCR EM ISOLADOS DE KLEBSIELLA PNEUMONIAE

open access: yesBrazilian Journal of Infectious Diseases, 2023
Introdução/Objetivo: Isolados de Klebsiella pneumoniae resistentes à multiplas drogas têm sido extensivamente reportados em todo o mundo. Alguns determinantes de resistência tornaram-se mais preocupantes pela sua disseminação, como o gene blakpc, já ...
Michelly Maria Pereira e Oliveira   +4 more
doaj   +1 more source

Optimising the Use of TRIzol-extracted Proteins in Surface Enhanced Laser Desorption/ Ionization (SELDI) Analysis [PDF]

open access: yes, 2006
Background Research with clinical specimens is always hampered by the limited availability of relevant samples, necessitating the use of a single sample for multiple assays. TRIzol is a common reagent for RNA extraction, but DNA and protein fractions can
Dang Tu   +11 more
core   +1 more source

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