Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology
Acta Stomatologica Croatica, 2016 Objective of work: The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real ...
Mônica Ghislaine Oliveira Alves +9 more
doaj +1 more source
Comparative analysis of the toxicity of gold nanoparticles in zebrafish [PDF]
, 2018 The use of nanoparticles - particles that range in size from 1 to 100 nanometres - has become increasingly prevalent in recent years, bringing with it a variety of potential toxic effects.
Abdal Dayem +31 more
core +3 more sources
Alkaline water modules the expression of inflammation genes: in vitro model for cancer prevention [PDF]
World Cancer Research Journal, 2020 OBJECTIVE: Many researches focus their attention on the behavior of cells to specific substances with alkalizing power. Each substance that we introduce with food, as well as water, contributes to conditioning the electrophilic balance (acid-base) of the
A. Del Buono +5 more
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pH-sensing G protein-coupled orphan receptor GPR68 is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progression [PDF]
PeerJ, 2023 Background Osteoarthritis (OA) is a debilitating joints disease affecting millions of people worldwide. As OA progresses, chondrocytes experience heightened catabolic activity, often accompanied by alterations in the extracellular environment’s ...
Nazir M. Khan +5 more
doaj +2 more sources
Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer
Biomedicines, 2021 MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis.
Luis Javier Martínez-González +8 more
doaj +1 more source
Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell Cultures
Methods and Protocols, 2021 Given that the airway epithelium is the initial site of infection, study of primary human airway epithelial cells (AEC) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) will be crucial to improved understanding of viral entry ...
Kaitlyn A. Barrow +6 more
doaj +1 more source
eEF1Bγ binds the Che-1 and TP53 gene promoters and their transcripts [PDF]
, 2016 Background: We have previously shown that the eukaryotic elongation factor subunit 1B gamma (eEF1Bγ) interacts with the RNA polymerase II (pol II) alpha-like subunit “C” (POLR2C), alone or complexed, in the pol II enzyme. Moreover, we demonstrated that
Ammassari-teule, Martine +9 more
core +1 more source
Memorias do Instituto Oswaldo Cruz, 2010 This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies.
Cleiton Paulo Aigner +5 more
doaj +1 more source
Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis
MethodsX, 2019 Dirofilaria immitis, the etiologic agent of canine heartworm disease, like several other filarial nematodes, harbors the bacterial endosymbiont Wolbachia. To investigate metabolic and functional pathways of D.
Chiara Lucchetti +5 more
doaj +1 more source
Epigallocatechin-3-Gallate (EGCG) Suppresses Pancreatic Cancer Cell Growth, Invasion, and Migration partly through the Inhibition of Akt Pathway and Epithelial-Mesenchymal Transition: Enhanced Efficacy when Combined with Gemcitabine. [PDF]
, 2019 Most pancreatic cancers are usually diagnosed at an advanced stage when they have already metastasized. Epigallocatechin-3-gallate (EGCG), a major polyphenolic constituent of green tea, has been shown to reduce pancreatic cancer growth, but its effect on
Hackman, Robert M +4 more
core +2 more sources