Results 11 to 20 of about 108,296 (191)

pH-sensing G protein-coupled orphan receptor GPR68 is expressed in human cartilage and correlates with degradation of extracellular matrix during OA progression [PDF]

open access: yesPeerJ, 2023
Background Osteoarthritis (OA) is a debilitating joints disease affecting millions of people worldwide. As OA progresses, chondrocytes experience heightened catabolic activity, often accompanied by alterations in the extracellular environment’s ...
Nazir M. Khan   +5 more
doaj   +2 more sources

Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

open access: yesBiomedicines, 2021
MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis.
Luis Javier Martínez-González   +8 more
doaj   +1 more source

Inactivation of Material from SARS-CoV-2-Infected Primary Airway Epithelial Cell Cultures

open access: yesMethods and Protocols, 2021
Given that the airway epithelium is the initial site of infection, study of primary human airway epithelial cells (AEC) infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) will be crucial to improved understanding of viral entry ...
Kaitlyn A. Barrow   +6 more
doaj   +1 more source

Real-time PCR-based quantification of Toxoplasma gondii in tissue samples of serologically positive outdoor chickens

open access: yesMemorias do Instituto Oswaldo Cruz, 2010
This study aimed to quantify Toxoplasma gondii in tissue samples of serologically positive chickens using real-time polymerase chain reaction (PCR). Of 65 chickens evaluated, 28 were positive for T. gondii antibodies.
Cleiton Paulo Aigner   +5 more
doaj   +1 more source

Optimized protocol for DNA/RNA co-extraction from adults of Dirofilaria immitis

open access: yesMethodsX, 2019
Dirofilaria immitis, the etiologic agent of canine heartworm disease, like several other filarial nematodes, harbors the bacterial endosymbiont Wolbachia. To investigate metabolic and functional pathways of D.
Chiara Lucchetti   +5 more
doaj   +1 more source

Extractions of High Quality RNA from the Seeds of Jerusalem Artichoke and Other Plant Species with High Levels of Starch and Lipid

open access: yesPlants, 2013
Jerusalem artichoke (Helianthus tuberosus L.) is an important tuber crop. However, Jerusalem artichoke seeds contain high levels of starch and lipid, making the extraction of high-quality RNA extremely difficult and the gene expression analysis ...
Tanupat Mornkham   +5 more
doaj   +1 more source

GENES DE REFERÊNCIA PARA ESTUDOS DE EXPRESSÃO RELATIVA POR QPCR EM ISOLADOS DE KLEBSIELLA PNEUMONIAE

open access: yesBrazilian Journal of Infectious Diseases, 2023
Introdução/Objetivo: Isolados de Klebsiella pneumoniae resistentes à multiplas drogas têm sido extensivamente reportados em todo o mundo. Alguns determinantes de resistência tornaram-se mais preocupantes pela sua disseminação, como o gene blakpc, já ...
Michelly Maria Pereira e Oliveira   +4 more
doaj   +1 more source

Isolation and solubilization of proteins after TRIzol® extraction of RNA and DNA from patient material following prolonged storage

open access: yesBioTechniques, 2007
A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic ...
Amanda B. Hummon   +3 more
doaj   +1 more source

Comparación de métodos de extracción de RNA para la detección por RT-PCR del Potato yellow vein virus (PYVV) en diferentes órganos de Solanum tuberosum Grupo Phureja

open access: yesRevista Colombiana de Biotecnología, 2013
Título en ingles: Comparison of RNA isolation methods for Potato yellow vein virus (PYVV) detection by RT-PCR in different organs of Solanum tuberosum Group Phureja Título corto: comparación de métodos de extracción de RNA Resumen Potato yellow vein ...
Anngie Katherine Hernández Guzmán   +1 more
doaj   +2 more sources

Gene expression profiling of whole blood: A comparative assessment of RNA-stabilizing collection methods.

open access: yesPLoS ONE, 2019
Peripheral Blood gene expression is widely used in the discovery of biomarkers and development of therapeutics. Recently, a spate of commercial blood collection and preservation systems have been introduced with proprietary variations that may ...
Duncan E Donohue   +8 more
doaj   +1 more source

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