Results 71 to 80 of about 113,418 (280)

Establishment and optimization of the two‐step induction system for generating primordial germ cell‐like cells from chicken embryonic stem cells

FEBS Open Bio, EarlyView.
This study optimizes a two‐step induction system to generate PGCLCs from chicken ESCs. The induced cells express germline markers and migrate to gonads in ovo, offering a novel approach for avian genetic resource conservation. Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs.
Zeyu Li, XianShuai Xu, GuangZheng Liu, XiaoQian Lv, JiuZhou Song, HongYan Sun, YingJie Niu, QiSheng Zuo, Wei Han, BiChun Li, Kai Jin   +10 more
wiley   +1 more source

Nucleic acids in inclusion bodies obtained from E. coli cells expressing human interferon-gamma

Microbial Cell Factories, 2020
Background Inclusion bodies (IBs) are protein aggregates in recombinant bacterial cells containing mainly the target recombinant protein. Although it has been shown that IBs contain functional proteins along with protein aggregates, their direct ...
Elena Krachmarova, Ivan Ivanov, Genoveva Nacheva   +2 more
doaj   +1 more source

Incubation temperature affects type X collagen expression in embryonic ducks [PDF]

, 2014
Optimal temperature regulation is an important component of avian incubation and the process of embryonic development can be accelerated by even small increases in incubation temperature.
Prickett, Amanda L.
core  

Diabetes‐induced vascular calcification is associated with low pyrophosphate and its oral supplementation prevents calcification in diabetic mice

FEBS Open Bio, EarlyView.
Induction of diabetes in three different mouse strains uniformly resulted in an increase in TNAP activity and a reduction in pyrophosphate (PPi) in the circulation. Inhibition of TNAP restored plasma PPi. Diabetes‐induced calcification in the media layer of the aorta was detected only in the Abcc6−/− strain, which is predisposed to ectopic ...
Krisztina Fülöp, Eszter Kozák, Natália Tőkési, Lilian Kocsis, Anikó Kelemen, Zsuzsanna Geszti, Adriána Kutás, Mariann Harangi, Ágnes Diószegi, Zsolt Rapi, József Balla, Olivier Le Saux, András Váradi, Viola Pomozi   +13 more
wiley   +1 more source

Conservation of Arabidopsis thaliana photoperiodic flowering time genes in onion (Allium cepa L.) [PDF]

, 2010
The genetics underlying onion development is poorly understood. Here the characterisation of onion homologues of Arabidopsis photoperiodic flowering pathway genes is reported with the end goal of accelerating onion breeding programmes by understanding ...
Massiah, Andrea J., Taylor, Andrew, Thomas, Brian   +2 more
core   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples

Brazilian Journal of Infectious Diseases
The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil.
Vanessa Salete de Paula, Livia Melo Villar, Ana Maria Coimbra Gaspar   +2 more
doaj   +1 more source

Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays

Journal of Infectious Diseases, 2020
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease.
Edward I. Patterson, T. Prince, Enyia R. Anderson, Aitor Casas-Sánchez, Shirley L. Smith, Cintia Cansado-Utrilla, T. Solomon, M. Griffiths, Á. Acosta-Serrano, L. Turtle, G. Hughes   +10 more
semanticscholar   +1 more source

Generation and characterization of a human iPSC line from a patient with propionic acidemia due to defects in the PCCA gene [PDF]

, 2017
Human induced pluripotent stem cell (iPSC) line was generated from fibroblasts of a patient with propionic acidemia carrying mutations in the PCCA gene: c.1899+4_1899+7delAGTA; p.(Cys616_Val633del) and c.1430 −−?_1643+?del; p.(Gly477Glufs*9 ...
Alonso-Barroso, Esmeralda, Brasil, Sandra, Briso-Montiano, Álvaro, Desviat, Lourdes R., Navarrete, Rosa, Pérez, Belén, Pérez-Cerdá, Celia, Richard, Eva, Ugarte, Magdalena   +8 more
core   +3 more sources

NR4A1 Exerts Pro‐Tumor Role in Glioblastoma via Inducing xCT/GPX4‐Regulated Ferroptosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Purpose This study investigates NR4A1's paradoxical roles in glioblastoma (GBM) progression, focusing on its mechanistic link to ferroptosis regulation. We aimed to resolve conflicting reports of NR4A1 as both an oncogene and a tumor suppressor by defining its transcriptional control over xCT/GPX4‐mediated iron homeostasis and its clinical ...
Peng Tao, Shikuan Din, Zhengkang Fu, Qian Sun, Xiwei Zhu, Yuxin Wei, Huan Qu, Xinyi Zhang, Jiaxuan Liu, Chuhua Fu, Qianxue Chen   +10 more
wiley   +1 more source