Results 91 to 100 of about 110,116 (296)

Interplay Between tRNA Modifications and Processing

Journal of Molecular Biology
Transfer RNAs play a key role during protein synthesis by decoding genetic information at the translating ribosome. During their biosynthesis, tRNA molecules undergo numerous processing steps. Moreover, tRNAs represent the RNA class that carries the largest variety and highest relative number of chemical modifications.
Jirka, Peschek, Francesca, Tuorto
openaire   +2 more sources

Versatile CRISPR‐Cas Tools for Gene Regulation in Zebrafish via an Enhanced Q Binary System

Advanced Science, EarlyView.
This study introduces CRISPR‐Q, a transgenic CRISPR‐Cas system leveraging the QFvpr/QUAS binary expression platform in zebrafish. CRISPR‐Q overcomes previous challenges in achieving stable and efficient gene regulation. By enabling precise spatiotemporal control of transcript knockdown (CRISPR‐QKD) and gene activation (CRISPR‐Qa), it provides a ...
Miaoyuan Shi, Weiqi Ge, Changheng Li, Bin Liu, Xiaoyi Deng, Chengjie Liu, Meijun Zheng, Pu Zhang, Lei Li, Ying Guo, Yunqi Han, Yu Yang, Yanxun V. Yu, Youngnam N. Jin   +13 more
wiley   +1 more source

Structures of KEOPS bound to tRNA reveal functional roles of the kinase Bud32

Nature Communications
The enzyme complex KEOPS (Kinase, Endopeptidase and Other Proteins of Small size) installs the universally conserved and essential N6-threonylcarbamoyl adenosine modification (t6A) on ANN-decoding tRNAs in eukaryotes and in archaea.
Samara Mishelle Ona Chuquimarca, Jonah Beenstock, Salima Daou, Jennifer Porat, Alexander F. A. Keszei, Jay Z. Yin, Tobias Beschauner, Mark A. Bayfield, Mohammad T. Mazhab-Jafari, Frank Sicheri   +9 more
doaj   +1 more source

Protein Phosphatase Sit4 Affects Lipid Droplet Synthesis and Soraphen A Resistance Independent of Its Role in Regulating Elongator Dependent tRNA Modification

Biomolecules, 2018
The protein phosphatase Sit4 has been shown to be required for lipogenesis and resistance against the acetyl-CoA carboxylase inhibitor soraphen A.
Bruno Leonardo Bozaquel-Morais, Leonie Vogt, Valentina D’Angelo, Raffael Schaffrath, Roland Klassen, Mónica Montero-Lomelí   +5 more
doaj   +1 more source

Targeting mitochondrial and cytosolic substrates of TRIT1 isopentenyltransferase: Specificity determinants and tRNA-i6A37 profiles.

PLoS Genetics, 2020
The tRNA isopentenyltransferases (IPTases), which add an isopentenyl group to N6 of A37 (i6A37) of certain tRNAs, are among a minority of enzymes that modify cytosolic and mitochondrial tRNAs.
Abdul Khalique, Sandy Mattijssen, Alexander F Haddad, Shereen Chaudhry, Richard J Maraia   +4 more
doaj   +1 more source

tRNA modifications tune m6A-dependent mRNA decay

Cell
Chemically modified nucleotides in mRNA are critical regulators of gene expression, primarily through interactions with reader proteins that bind to these modifications. Here, we present a mechanism by which the epitranscriptomic mark N6-methyladenosine (m6A) is read by tRNAs during translation.
Bastian Linder, Puneet Sharma, Jie Wu, Tosca Birbaumer, Cristian Eggers, Shino Murakami, Roman E. Ott, Kai Fenzl, Hannah Vorgerd, Florian Erhard, Samie R. Jaffrey, Sebastian A. Leidel, Lars M. Steinmetz   +12 more
openaire   +2 more sources

APOE‐stratified Proteomic and Metabolomic Analysis Reveals Mitochondrial Dysfunction Inflammation and Lipid Dysregulation in Alzheimer's Disease

Advanced Science, EarlyView.
A large‐scale multiomic dataset (proteomic and metabolomic) comprising 3,060 plasma samples were analyzed to identify proteins, metabolites, pathways, and protein‐associated drugs linked to Alzheimer’s Disease (AD) independently of apolipoprotein E (APOE). AD was associated with a distinct molecular signature that captures.
Fuhai Li, Yike Chen, Daniel Western, Muhammad Ali, Menghan Liu, Katherine Gong, Ying Xu, Joseph Lowery, David M. Holtzman, Chloe Robins, John D. Eicher, Yen‐Ning Huang, ShiWei Liu, Tamina Park, Andrew J. Saykin, Kwangsik Nho, Mahdi Moqri, Richard C. Mohs, Amelia Farinas, Patricia Moran‐Losada, Hamilton Se‐Hwee Oh, Tony Wyss‐Coray, Carlos Cruchaga   +22 more
wiley   +1 more source

Posttranscriptional modification of tRNA in thermophilic archaea (Archaebacteria) [PDF]

Journal of Bacteriology, 1991
Nucleoside modification has been studied in unfractionated tRNA from 11 thermophilic archaea (archaebacteria), including phylogenetically diverse representatives of thermophilic methanogens and sulfur-metabolizing hyperthermophiles which grow optimally in the temperature range of 56 (Thermoplasma acidophilum) to 105 degrees C (Pyrodictium occultum ...
C G, Edmonds, P F, Crain, R, Gupta, T, Hashizume, C H, Hocart, J A, Kowalak, S C, Pomerantz, K O, Stetter, J A, McCloskey   +8 more
openaire   +2 more sources

Programmable Klebsiella pneumoniae Phage Tropism Enabled by Scalable Receptor‐Binding Protein Mining and Modular Assembly

Advanced Science, EarlyView.
This work introduces a scalable, data‐driven strategy that turns the vast sequence diversity of phage receptor‐binding proteins into modular tools for engineering customizable phages. By clustering and experimentally validating diverse RBPs, the authors build an accurate map linking sequence to host specificity and create plug‐and‐play modules that ...
Shisong Jing, Yiyao Song, Xianbiao Bi, Yuqin Song, Dawei Wei, Jiangqing Huang, Yuan Zeng, Gang Zhang, Rong Zhang, Chao Wang, Jie Feng   +10 more
wiley   +1 more source

Recent Advances in Our Understanding of the Biosynthesis of Sulfur Modifications in tRNAs

Frontiers in Microbiology, 2018
Sulfur is an essential element in all living organisms. In tRNA molecules, there are many sulfur-containing nucleosides, introduced post-transcriptionally, that function to ensure proper codon recognition or stabilization of tRNA structure, thereby ...
Naoki Shigi
doaj   +1 more source