Results 71 to 80 of about 764 (211)

Heat stress promotes pre-tRNA capping to modulate cap-dependent translation in Saccharomyces cerevisiae

Nature Communications
Methylguanosine capping, a post-transcriptional modification of the 5′ terminus of RNA polymerase II transcripts, influences RNA function, stability, and protein synthesis.
Ittoku Kikuchi, Takayuki Ohira, Taichi Nakatsuka, Yuma Ishigami, Tsutomu Suzuki   +4 more
doaj   +1 more source

Nuclear-encoded factors involved in post-transcriptional processing and modification of mitochondrial tRNAs in human disease

Frontiers in Genetics, 2015
The human mitochondrial genome (mtDNA) encodes twenty-two tRNAs (mt-tRNAs) that are necessary for the intraorganellar translation of the thirteen mtDNA-encoded subunits of the mitochondrial respiratory chain complexes.
Christopher A Powell, Thomas J Nicholls, Michal eMinczuk   +2 more
doaj   +1 more source

PEAR1 Promotes Glucose Metabolism Reprogramming in Sepsis‐Associated Acute Lung Injury via AARS1‐Mediated HIF‐1α Lactylation

Advanced Science, EarlyView.
This study revealed that a PEAR1/HIF‐1α/ glycolysis/lactate/H3K18la positive feedback loop in PMVECs that drives the development of S‐ALI. Mechanistically, PEAR1 mediates the binding of HIF‐1α to AARS1, leading to the lactylation of HIF‐1α, the primary lactylation site of which is K172.
Shuai Li, Zongqing Lu, Pu Fang, Qingqing Pang, Xianghui Wu, Bo Wang, Min Liang, Jingjing Pan, Haobo Kong, Haijian Cai, Juanjuan Li, Jiaying Kang, Dongsheng Wang, Dunling Xia, Mengmeng Xu, Qinghai You   +15 more
wiley   +1 more source

De Novo Discovery of Nonstandard Thioisoindole‐Bridged Bicyclic Peptides Targeting Traf2‐ and NCK‐Interacting Kinase

Angewandte Chemie, EarlyView.
An optimized strategy for the ribosomal synthesis of thioisoindole‐bridged bicyclic (TiB) peptides is incorporated into the RaPID platform, enabling high‐throughput discovery from topologically defined TiB libraries. Proof‐of‐concept selection against TNIK identifies nanomolar‐affinity ligands with inhibitory activity, while x‐ray crystallography ...
Yue Zhang, Alexander A. Vinogradov, Keisuke Hamada, Yin Sun, Toru Sengoku, Hiroaki Suga   +5 more
wiley   +2 more sources

Emerging roles of tRNA modification-mediated codon-specific translational reprogramming in cancer biology

Cell Death and Disease
Cancer has become a leading cause of mortality worldwide, with alarming increases in incidence and mortality rates. Emerging evidence suggests that tRNA modification enzymes play a crucial role in cancer development by modulating codon-specific ...
Hanwei Wang, Junsi Zhang, Cen Jiang, Sunwang Xu   +3 more
doaj   +1 more source

A Universal Protein Ladder for Standardization of Diverse FRET Assays

Advanced Science, EarlyView.
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith, Katie L. Gelder, Lewis Hunter‐Craig, Daniel A. Bose, Timothy D. Craggs, Alison E. Twelvetrees   +5 more
wiley   +1 more source

tRNA m1A modification ensures HSPC production via modulating Nrf1 translation in zebrafish

EMBO Reports
During embryogenesis, nascent hematopoietic stem and progenitor cells (HSPCs) arise from hemogenic endothelium via endothelial-to-hematopoietic transition (EHT).
Zhenkun Dong, Panfeng Li, Mengyao Liu, Sifeng Wang, Weiyi Lai, Yining Liu, Hailin Wang, Ang Li, Lu Wang   +8 more
doaj   +1 more source

A Second Pathogenic Protein, PolyGN2C‐iso2, Reveals a Dual‐Protein Pathology in Neuronal Intranuclear Inclusion Disease

Advanced Science, EarlyView.
This study reveals that NOTCH2NLC transcript variant 2 generates PolyGN2C‐iso2, an aggregating protein present within intranuclear inclusions of NIID patient tissues. A novel mouse model expressing PolyGN2C‐iso2 recapitulates white matter abnormalities and cognitive deficits, mechanistically linked to mitochondrial dysfunction. These findings support a
Kang Zhang, Wenhao Ma, Yi Zhou, Zhijie Wu, Pan Gao, Hongze Niu, Hongfei Tai, Tianyi Zhao, Zheyue Dong, Li Li, Yan Zhang, An Wang, Si Shen, Yueyang Li, Sifei Yu, Yan Peng, Wang Sheng, Xiaoyan Dong, Hua Pan, Kaibin Shi, Magdalena J. Koziol, Xiaobing Wu, Zaiqiang Zhang   +22 more
wiley   +1 more source

Beyond Simple Mimicry: Next‐Generation Geometric Architectures and Future Paradigms in Small‐Molecule and Macrocyclic Peptidomimetics

Angewandte Chemie, EarlyView.
Peptide‐to‐Small Molecule Paradigm: Peptidomimetics have evolved from simple mimicry toward drug‐like scaffolds supported by increasing clinical successes. This Perspective highlights the geometric design principles—linear repetition, convergent fusion, and cyclization—defining the next‐generation peptidomimetic architectures capable of targeting ...
Jesang Lee, Sumin Son, Jeong Yeon Yoo, Ji Hyae Lee, Meehyun Chun, Seung Bum Park   +5 more
wiley   +2 more sources

Protein Phosphatase Sit4 Affects Lipid Droplet Synthesis and Soraphen A Resistance Independent of Its Role in Regulating Elongator Dependent tRNA Modification

Biomolecules, 2018
The protein phosphatase Sit4 has been shown to be required for lipogenesis and resistance against the acetyl-CoA carboxylase inhibitor soraphen A.
Bruno Leonardo Bozaquel-Morais, Leonie Vogt, Valentina D’Angelo, Raffael Schaffrath, Roland Klassen, Mónica Montero-Lomelí   +5 more
doaj   +1 more source