Results 91 to 100 of about 415,043 (258)

Successful cell culture isolation and experimental pathogenicity evaluation of porcine bocavirus G3

Virulence
Since its initial discovery in Swedish pigs in 2009, porcine bocavirus (PBoV) has been detected across Asia, Europe, Africa, and North America. However, the pathogenic potential of PBoV has remained uncertain due to the lack of suitable cell culture ...
Yuli Hu, Juanjuan Min, Huan Xu, Lingfeng Li, Ying Shi, Haichao Wu, Jia Zheng, Ningning Fu, Min Xu, Jianfu Sun, Yushuang Zhou, Jinhua Chen, Taotao Yang, Wen Sun   +13 more
doaj   +1 more source

Interconnected Porous Hydrogels with Tunable Anisotropy Through Aqueous Emulsion Bioprinting

Advanced Functional Materials, EarlyView.
A 3D bioprintable microporous bioink is developed using an aqueous two‐phase system (ATPS) composed of extracellular matrix (ECM) mimetic biopolymers. The ATPS bioink enables the fabrication of interconnected porous architectures with up to 70% porosity, supporting long‐term cell viability and 3D cell alignment, enabling a simultaneous generation of ...
Hugo Edgar‐Vilar, Francisca P. Correia, Inês M. Freitas, Vítor M. Gaspar, João F. Mano   +4 more
wiley   +1 more source

Bio‐Orthogonally Crosslinked Supramolecular Polymer Bottlebrush Hydrogels for Long‐Term 3D Cell Culture

Advanced Functional Materials, EarlyView.
Fibrous benzenetrispeptide (BTP) hydrogels, fabricated via strain‐promoted azide‐alkyne cycloaddition (SPAAC) crosslinking, form robust, bioinert networks. These hydrogels can support 3D cell culture, where cell viability and colony growth depend on the fiber content.
Ceren C. Pihlamagi, David Pretzel, Peer Nölte, Kourosh Rezaei, Ulrich S. Schubert, Johannes C. Brendel   +5 more
wiley   +1 more source

A Model of Demasking and Hydrolysis of Peptide Bonds During Tryptic Digestion of β-Casein and β-Lactoglobulin

Molecules
The prediction of polypeptide chain fragmentation during digestion (proteolysis) of protein substrates by trypsin was carried out for globular β-lactoglobulin (β-LG) and micellar β-casein (β-CN).
Mikhail M. Vorob’ev
doaj   +1 more source

Trypsinized suction blister

Journal of Cutaneous and Aesthetic Surgery
Suction blister epidermal grafting (SBEG) is a standard technique for treating vitiligo on challenging areas like fingertips and lips but often results in pigment halos and difficulties with small, curved surfaces. We propose a modified SBEG technique, utilizing trypsinization of one blister graft to improve melanocyte spread and reduce peri-graft halo.
Patra, Suman, Kumar, Shubham, Kaur, Maninder   +2 more
openaire   +2 more sources

Engineering a Sonotherapeutic RBC Membrane‐Derived Nanoparticle Platform for the Treatment of Liver Cancer

Advanced Functional Materials, EarlyView.
Herein, an RBC membrane‐derived nanoparticle (CMN‐ICG) is engineered to efficiently deliver a sonosensitizing agent, indocyanine green (ICG), for sonotherapy of hepatocellular carcinoma (HCC). CMN‐ICG exhibits excellent cytocompatibility, significantly enhances hepatocyte uptake, and produces excessive reactive oxygen species (ROS) upon ultrasound ...
Alap Ali Zahid, Aishik Chakraborty, Wei Luo, Juhi Bora, John Joseph Kelly, John Andrew Ronald, Arghya Paul   +6 more
wiley   +1 more source

Biochemical characterization of trypsin from Indonesian skipjack tuna (Katsuwonus pelamis) viscera

Open Agriculture
Trypsin production from skipjack tuna (Katsuwonus pelamis) viscera is one significant way to increase the value of fish’s industrial waste. The present work reports the biochemical properties of trypsin from skipjack tuna viscera.
Dali Faiza A., Nurjanah Nurjanah, Lioe Hanifah N., Suhartono Maggy T.   +3 more
doaj   +1 more source

Research progress of porcine epidemic diarrhea virus S protein

Frontiers in Microbiology
Porcine epidemic diarrhea virus (PEDV) is a single-stranded RNA virus with a capsid membrane that causes acute infectious gastrointestinal disease characterized by vomiting, diarrhea, and dehydration in swine.
Haojian Luo, Zhaoping Liang, Junjie Lin, Yiqiao Wang, Yingying Liu, Kun Mei, Mengmeng Zhao, Shujian Huang, Shujian Huang   +8 more
doaj   +1 more source

PRELIVE: A Framework for Predicting Lipid Nanoparticles In Vivo Efficacy and Reducing Reliance on Animal Testing

Advanced Functional Materials, EarlyView.
PREdicting LNP In Vivo Efficacy (PRELIVE) framework enables the prediction of lipid nanoparticle (LNPs) organ‐specific delivery through dual modeling approaches. Composition‐based models using formulation parameters and protein corona‐based models using biological fingerprints both achieve high predictive accuracy across multiple organs.
Belal I. Hanafy, Chuan‐En Lu, Kai Liu, Audrey Gallud   +3 more
wiley   +1 more source

Gasdermin D‐Mediated Pyroptosis Exerts Two Opposite Effects of Resisting Enzymatic Digestion and Expanding Inflammatory Response in Acute Pancreatitis

Advanced Science
Gasdermin D (GSDMD)‐induced pyroptosis is associated with inflammatory disease. However, the role of GSDMD in acute pancreatitis (AP) is not yet fully elucidated.
Chaoxu Liu, Ning Liang, Chen Wu, Zhaoyan Qiu, Qian Huang, Xiaolong Wei, Shurong Zhang, Shuanghong Lei, Tao Yang, Gang Wang, Qian Wang   +10 more
doaj   +1 more source