Results 81 to 90 of about 674,005 (346)

Advances in Analysis of Milk Proteases Activity at Surfaces and in a Volume by Acoustic Methods

Sensors, 2020
This review is focused on the application of surface and volume-sensitive acoustic methods for the detection of milk proteases such as trypsin and plasmin. While trypsin is an important protein of human milk, plasmin is a protease that plays an important
Mark Dizon, Marek Tatarko, Tibor Hianik
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Investigation of Mechanisms of Interaction of Yersinia pestis EV NIIEG Vaccine Strain Bacteria with Human Red Blood Cells

Проблемы особо опасных инфекций, 2012
The comparative experiments demonstrated strong correlation between the index of Yersinia pestis EV NIIEG strain adhesion to human red blood cells and hydrophobic properties of bacteria.
E. V. Pimenov, V. A. Oborin, A. G. Ivonin   +2 more
doaj   +1 more source

Intracapillary leucocyte accumulation as a novel antihaemorrhagic mechanism in acute pancreatitis in mice [PDF]

, 2009
Background: Pancreatic infiltration by leucocytes represents a hallmark in acute pancreatitis. Although leucocytes play an active role in the pathophysiology of this disease, the relation between leucocyte activation, microvascular injury and haemorrhage
Deduchovas, O., Hartwig, W., Kerkadze, V., Märten, A., Parseliunas, A., Ryschich, E., Salnikova, O., Schmidt, J., Sperandio, Markus   +8 more
core   +1 more source

Discovery and Targeted Proteomic Studies Reveal Striatal Markers Validated for Huntington's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Clinical trials for Huntington's disease (HD) enrolling persons before clinical motor diagnosis (CMD) lack validated biomarkers. This study aimed to conduct an unbiased discovery analysis and a targeted examination of proteomic biomarkers scrutinized by clinical validation. Methods Cerebrospinal fluid was obtained from PREDICT‐HD and
Daniel Chelsky, Cara Joyce, H. Jeremy Bockholt, Paul A. Rudnick, William H. Adams, Fiona McAllister, Justin W. Smock, Michael A. Newton, Jane S. Paulsen   +8 more
wiley   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Atividade da tripsina em rã-touro na fase pós-metamórfica Trypsin activity of the bullfrog in the post-metamorphic phase

Revista Brasileira de Zootecnia, 2004
Com o objetivo de avaliar a atividade enzimática da tripsina no conteúdo intestinal da rã-touro (Rana catesbeiana Shaw, 1802), 320 animais com peso médio de 3,6 g foram distribuídos em baias-teste com temperatura e fotoperíodo controlados.
Luís Gustavo Tavares Braga, Maria Goreti de Almeida Oliveira, William Cardoso Lima, Ricardo Frederico Euclydes   +3 more
doaj   +1 more source

HPLC-MS characterisation of chelate modified somatropin [PDF]

, 2010
Somatropin is a recombinant human growth hormone, consisting of 191 amino acids. This protein is clinically used in children and adults with inadequate endogenous growth hormone to stimulate a normal bone and muscle growth.
De Spiegeleer, Bart, Pauwels, Ewald, Van De Wiele, Christophe, Van Dorpe, Sylvia, Vergote, Valentijn, Wynendaele, Evelien   +5 more
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