Transcriptomic Responses to Different Cry1Ac Selection Stresses in Helicoverpa armigera
Frontiers in Physiology, 2018 Helicoverpa armigera can develop resistance to Bacillus thuringiensis (Bt), which threaten the long-term success of Bt crops. In the present study, RNAseq was employed to investigate the midgut genes response to strains with different levels of ...
Jizhen Wei +6 more
doaj +1 more source
Microorganisms, 2023 Biofilms consist of microbial communities enclosed in a self-produced extracellular matrix which is mainly responsible of biofilm virulence. Targeting this matrix could be an effective strategy to control biofilms.
Samah Mechmechani +6 more
doaj +1 more source
Multisecond ligand dissociation dynamics from atomistic simulations [PDF]
Nat. Commun. 11, 2918 (2020), 2020 Coarse-graining of fully atomistic molecular dynamics simulations is a long-standing goal in order to allow the description of processes occurring on biologically relevant timescales. For example, the prediction of pathways, rates and rate-limiting steps in protein-ligand unbinding is crucial for modern drug discovery. To achieve the enhanced sampling,
arxiv +1 more source
Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms [PDF]
, 2011 Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/α-amylase inhibitors are two of the best characterized protease inhibitor families in plants.
Cambra Marin, Ines +5 more
core +2 more sources
Advances in Colorimetric Assay Based on AuNPs Modified by Proteins and Nucleic Acid Aptamers
Chemosensors, 2021 This review is focused on the biosensing assay based on AuNPs (AuNPs) modified by proteins, peptides and nucleic acid aptamers. The unique physical properties of AuNPs allow their modification by proteins, peptides or nucleic acid aptamers by ...
Sopio Melikishvili +2 more
doaj +1 more source
Sensitivity of protein rigidity analysis to small structural variations: a large-scale comparative analysis [PDF]
physicalbiology 6, 046005-11 (2009), 2008 Rigidity analysis using the "pebble game" can usefully be applied to protein crystal structures to obtain information on protein folding, assembly and the structure-function relationship. However, previous work using this technique has not made clear how sensitive rigidity analysis is to small structural variations.
arxiv +1 more source
RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of Grapholita molesta
Insects, 2022 Grapholita molesta is an important fruit tree worldwide pest which feeds on hosts extensively and does serious harm. In this paper, the growth and development parameters and protease activities of G. molesta fed on different hosts were compared.
Dongbiao Lü +7 more
doaj +1 more source
Specificity of Trypsin and Chymotrypsin: Loop Motion Controlled Dynamic Correlation as a Determinant [PDF]
, 2005 Trypsin and chymotrypsin are both serine proteases with high sequence and structural similarities, but with different substrate specificity. Previous experiments have demonstrated the critical role of the two loops outside the binding pocket in controlling the specificity of the two enzymes.
arxiv +1 more source
Recent advances in computational methods for studying ligand binding kinetics [PDF]
arXiv, 2022 Binding kinetic parameters can be correlated with drug efficacy, which led to the development of various computational methods for predicting binding kinetic rates and gaining insight into protein-drug binding paths and mechanisms in recent years. In this review, we introduce and compare computational methods recently developed and applied to two ...
arxiv
Markov modeling of peptide folding in the presence of protein crowders [PDF]
J. Chem. Phys. 148, 055101 (2018), 2018 We use Markov state models (MSMs) to analyze the dynamics of a $\beta$-hairpin-forming peptide in Monte Carlo (MC) simulations with interacting protein crowders, for two different types of crowder proteins [bovine pancreatic trypsin inhibitor (BPTI) and GB1].
arxiv +1 more source