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Avapritinib reduces symptoms and mast cell burden in systemic mastocytosis. [PDF]

open access: yesAllergy Asthma Clin Immunol
Nöldeke P   +4 more
europepmc   +1 more source
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Expression of α-tryptase and β-tryptase by human basophils

Journal of Allergy and Clinical Immunology, 2004
Background α and β-Tryptase levels in serum are clinical tools for the evaluation of systemic anaphylaxis and systemic mastocytosis. Basophils and mast cells are known to produce these proteins. Objective The current study examines the effect of the α,β-tryptase genotype on basophil tryptase levels and the type of tryptase stored in these cells ...
Lawrence B Schwartz
exaly   +2 more sources

Rat mast cell tryptase

Archives of Biochemistry and Biophysics, 1991
Rat mast cell tryptase is located largely if not totally in the cell's secretory granules. When the active site reagent [3H]diisopropyl fluorophosphate was used to label tryptase and chymase simultaneously, the ratio of tryptase:chymase active sites was determined to be 0.05. In comparison to chymase and tryptase in other species and chymase in the rat,
D, Lagunoff, A, Rickard, C, Marquardt
openaire   +2 more sources

BOVINE TRYPTASE: PURIFICATION AND CHARACTERIZATION

Biological Chemistry Hoppe-Seyler, 1992
Bovine tryptase, a mast cell trypsin-like protease, was isolated from liver capsula and from mast cells obtained from the same tissue. The purification procedure which leads to an increase in tryptase activity of 850 fold, involves high salt extraction, hydrophobic interaction chromatography on octyl-Sepharose and affinity chromatography on heparin ...
Fiorucci, L, Erba, F, Ascoli, F
openaire   +3 more sources

Human skin tryptase: purification, partial characterization and comparison with human lung tryptase

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1988
Human skin tryptase was isolated using stepwise low- and high-salt extraction and further purified 448-fold with 33% yield using octyl-Sepharose CL-4B hydrophobic affinity chromatography, Sephacryl S-200 gel filtration and finally octyl-Sepharose CL-4B or cellulose phosphate ion exchange chromatography.
I T, Harvima   +3 more
openaire   +2 more sources

Biomarkers of anaphylaxis, beyond tryptase

Current Opinion in Allergy & Clinical Immunology, 2015
The purpose of this study is to describe the current knowledge regarding mediators involved in anaphylactic reactions, with a special focus on key effector cells and mechanisms.New insight into the potential relevance of pathways other than mast cell degranulation has been unravelled, such as the role of cytokines, platelet activation factor, lipid ...
Anna, Sala-Cunill, Victoria, Cardona
openaire   +2 more sources

Expression of alpha-tryptase and beta-tryptase by human basophils.

The Journal of allergy and clinical immunology, 2004
Alpha and beta-tryptase levels in serum are clinical tools for the evaluation of systemic anaphylaxis and systemic mastocytosis. Basophils and mast cells are known to produce these proteins.The current study examines the effect of the alpha,beta-tryptase genotype on basophil tryptase levels and the type of tryptase stored in these cells.Tryptase ...
Sherryline, Jogie-Brahim   +4 more
openaire   +2 more sources

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