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Culture and Isolation of Brain Tumor Initiating Cells
Current Protocols in Stem Cell Biology, 2009AbstractThis unit describes protocols for the culture and isolation of brain tumor initiating cells (BTIC). The cancer stem cell (CSC) hypothesis suggests that tumors are maintained exclusively by a rare fraction of cells that have stem cell properties.
Nicole McFarlane +3 more
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Entosis and Cell Cycle in Tumor Cell Culture
Cell and Tissue Biology, 2019Entosis is a type of cell cannibalism during which one tumor cell invades another. The fate of the inner cell can vary. It can leave the entotic vacuole, divide within it, or be subjected to lysosome-mediated degradation. The aim of our work was to determine whether MCF7 (p53+) human breast adenocarcinoma cells and A431 (p53-) human epidermoid ...
G. E. Onishchenko +4 more
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Spontaneous cell shedding by tumor cells in monolayer culture
In Vitro Cellular & Developmental Biology, 1986Sarcoma 180 monolayers spontaneously shed single cells and small multicellular aggregates into the surrounding medium to produce a dual population of floating and substratum-attached cells. Shedding was a motility-associated event that occurred when cells attempted to migrate over one another.
James E. Thomas +2 more
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In vitro Culture of Ehrlich Ascites Tumor Cells
Science, 1960The successful culturing and subculturing of ascites tumor cells for 80 odd days are described, as well as the growth pattern of these cells during this period. The cytological appearance of cells after 3 weeks in tissue culture and reinoculation into mice is also presented.
Deschner, E E, Allen, B R
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Culturing breast tumor cells in the presence of hormones
Journal of Surgical Oncology, 1974AbstractA tissue culture technique for culturing human breast tumor cells was utilized to invesitgate the response of these cells to various hormones. Due to the small number of tumors investigated, no definite pattern of response was evident. However, the methods described may provide information which can be of useful clinical importance.
J. Mills +3 more
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Immunofluorescent studies of cultured canine tumor cells
International Journal of Cancer, 1972AbstractThe indirect immunofluorescent (IF) test was used in an immunological survey of 17 sera from tumor‐bearing and normal dogs. These sera were reacted against 33 cultured canine tumor and normal cells. A high percentage (70%) of IF positive reactions were seen in cells derived from sarcomatous tumors while only 4% of the cultures from tumor types ...
Wilna A. Woods +5 more
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Antibody transport in cultured tumor cell layers
Journal of Controlled Release, 1998This review summarizes our recent in vitro studies of the factors affecting the tumor penetration of immunoconjugates. The studies were designed to probe the mechanisms of diffusion and convection, using a cultured layer of mouse melanoma cells as a model tumor cell layer and an antibody to the murine transferrin receptor as a model ligand.
P. A. Kitos +4 more
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Confrontation responses of normal and tumorous cells in culture
Experimental Cell Research, 1970Abstract 1. 1. A line of normal rat fibroblasts (NRF) was established and a diploid clone was obtained (NRF-C-1). NRF-C-1 cells grew in parallel array as monolayers in tissue culture and did not form tumors on inoculation into isologous newborn rats. 2. 2.
Catherine Armstrong Reznikoff +1 more
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GROWTH OF EHRLICH ASCITES TUMOR CELLS IN TISSUE CULTURE
Canadian Journal of Biochemistry and Physiology, 1960Null
Jackson, P W, Giuffre, N, Perlman, D
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Effect of a hypoxic tumor cell cytotoxic disulfide on the membrane and DNA of tumor cells in culture
Anti-Cancer Drugs, 1992A disulfide, n-butyl 2-imidazolyl disulfide (III-2), recently reported to be more toxic to EMT6 tumor cells under hypoxia has also been shown to be preferentially toxic to KHT/iv cells under hypoxia with an IC90 value 3-fold lower versus that measured in air. The IC90 values for both cell lines were markedly affected when the pHe was decreased.
Kirkpatrick Dl +5 more
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