Results 121 to 130 of about 147,625 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Turning points in the history of  Nordic higher education

open access: yesKasvatus & Aika, 2009
An editorial written by visiting editor Pieter ...
Pieter Dhondt
doaj   +2 more sources

19th Century Turning Points in U.S. History: 1814-1826

open access: yes, 2008
1814 ? WASHINGTON DC IS CAPTURED AND BURNED BY THE BRITISH1814 ? STAR SPANGLED BANNER IS WRITTEN1815 ? BATTLE OF NEW ORLEANS1819 ? WASHINGTON IRVING PUBLISHES ?RIP VAN WINKLE?1821 ? INAUGURATION OF THE SANTA FE TRAIL1825 ?

core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Temps longs et temps courts dans les parcours de jeunes adultes en situation de précarité

open access: yesSociétés et Jeunesses en Difficulté, 2018
The decline of a homogenous social time in industrial societies opens up the way to a pluralization of social temporalities. A process recognizable in young adults’ life courses regarding the density of transitions encountered during their process to ...
Eddy Supeno, Sylvain Bourdon
doaj  

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

Home - About - Disclaimer - Privacy