Results 101 to 110 of about 6,969,201 (284)
ATZ‐1 promotes DNA replication efficiency to maintain normal meiotic function
FEBS Open Bio, EarlyView.Absence of ATZ‐1 interferes with meiotic DNA replication and cell cycle function via CHK‐1. This causes downstream defects associated with DNA damage and genomic integrity. Taken together, this study suggests that ATZ‐1 influences DNA replication efficiency and cell cycle function to maintain normal meiotic function.
Taylin E. Gourley +5 more
wiley +1 more source
Influence of Dispersant and Surfactant on nZVI Characterization by Dynamic Light Scattering
Engineering ProceedingsThe agrifood industries generate tremendous amounts of waste, with the valorization of these wastes being of the utmost importance. The aim of this work was to synthesize green zero-valent iron nanoparticles (nZVI) using hydromethanolic extracts of spent
Filipe Fernandes +3 more
doaj +1 more source
Tween-20 does not affect localization of RanGAP to the NPC.
, 2013 HeLa cells were incubated in serum-free DMEM (Untreated), serum-free DMEM containing 150 µM Tween-20 for 4 h (Tween-20), serum-free DMEM containing 150 µM Tween-20 for 4 h, washed and then placed in fresh serum-free DMEM for 1 h (Wash), or in serum-free ...
Jacqueline B. Pierce (312676) +2 more
core +1 more source
Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA
FEBS Open Bio, EarlyView.Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley +1 more source
Effect of the surfactants tween 20 and CTAB on fluoranthene and anthracene degradation by P. putida [PDF]
, 2001 The effect of a nonionic and a cationic surfactant, Tween 20 and CTAB, on the biodegradation rate of fluoranthene and anthracene by a P. putida strain was investigated. Tween 20 had a positive effect on the biodegradation rate of anthracene.
Melo, L. F. +2 more
core +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
, 1984 The effect of the nonionic detergent Tween 20 on background staining, sensitivity, and specificity in the indirect immunoperoxidase staining for blood group antigen A was investigated histologically and spectrophotometrically.
T Nørgaard, B R Juhl, O J Bjerrum
core +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source