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Two-Dimensional Difference Gel Electrophoresis
Nature Protocols, 2006The two-dimensional (2D) polyacrylamide gel-based approach to protein profiling has been successful because it is an accessible, inexpensive, and powerful tool for the analysis of global patterns of protein expression. All protein spots that are resolved and detected within the 104 to 105 dynamic range of gel capacity can be studied qualitatively and ...
Surya, Viswanathan +2 more
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Two-Dimensional Gel Electrophoresis
Science, 1962Improved resolution of serum protein mixtures is effected by electrophoresis, first in a 5 percent acrylamide gel following which a strip of the resolved pattern is embedded in 8 percent gel and subjected to a second electrophoresis separation at right angles to the first.
S, Raymond, B, Aurell
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Two-dimensional strandness-dependent electrophoresis
Nature Protocols, 2006Two-dimensional strandness-dependent electrophoresis (2D-SDE) separates nucleic acids in complex samples according to strandness, conformation and length. Under the non-denaturing conditions of the first electrophoretic step, single-stranded DNA, double-stranded DNA and RNA.DNA hybrids of similar length migrate at different rates.
Gudmundur H, Gunnarsson +4 more
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Two-Dimensional Difference Gel Electrophoresis
2018Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample are covalently tagged with different color fluorescent dyes that are designed to have no effect on the relative migration of proteins
Malachi, Blundon +4 more
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Two-dimensional electrophoresis computerized processing
International Journal of Biochemistry, 1988This paper describes various methods suitable for implementation of two-dimensional processing software. The different steps leading to a complete processing are described, from the digitalization of the image to the processing of the resulting data. The characteristics of a convenient digitalization system are discussed. The different software devoted
P, Vincens, P, Tarroux
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Two-dimensional electrophoresis
Nature, 1991Recent developments in the first- and second-dimension steps of two-dimensional electrophoresis have improved pattern reproducibility.
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ELECTROPHORESIS, 1996
AbstractWhile a two‐dimensional electrophoresis (2‐DE) database is a relatively old concept, in recent years it generated renewed interest within the 2‐DE community due to two main factors: (i) The high reproducibility of the current 2‐DE method allows 2‐DE images to be exchanged and compared between laboratories.
Appel, Ron David +6 more
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AbstractWhile a two‐dimensional electrophoresis (2‐DE) database is a relatively old concept, in recent years it generated renewed interest within the 2‐DE community due to two main factors: (i) The high reproducibility of the current 2‐DE method allows 2‐DE images to be exchanged and compared between laboratories.
Appel, Ron David +6 more
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High-Resolution Two-Dimensional Electrophoresis
2009Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry is currently the workhorse for the majority of ongoing proteome projects. Although alternative/complementary technologies, such as MudPIT, ICAT, or protein arrays, have emerged recently, there is up to now no ...
Walter, Weiss, Angelika, Görg
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Two-Dimensional Electrophoresis
1997In organelles, cells and tissues of all organisms complex functions and metabolic reactions are maintained by proteins within and at the surface of each compartment. The number of proteins occurring in a biological compartment corresponds to the complexity of the functions it has to fulfill.
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Two-dimensional electrophoresis image interpretation
IEEE Transactions on Biomedical Engineering, 1993A novel method to interpret two-dimensional electrophoresis gels is presented. Genetic background and electrophoretic processes are summarized. Present methods to analyze gel images and to exploit series of gels are described, then their drawbacks are outlined.
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