A semi-virtual two dimensional gel electrophoresis: IF–ESI LC-MS/MS [PDF]
MethodsX, 2017 A method for increasing the productivity of ESI LC-MS/MS (electrospray ionization-liquid chromatography-tandem mass spectrometry) was proposed and applied. After IF (isoelectric focusing) of the sample using IPG (immobilized pH gradient) strip, the strip
Stanislav Naryzhny +4 more
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Comparative Skeletal Muscle Proteomics Using Two-Dimensional Gel Electrophoresis [PDF]
Proteomes, 2016 The pioneering work by Patrick H. O’Farrell established two-dimensional gel electrophoresis as one of the most important high-resolution protein separation techniques of modern biochemistry (Journal of Biological Chemistry 1975, 250, 4007–4021).
Sandra Murphy +2 more
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Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome. [PDF]
PLoS ONE, 2019 The primary components of human hair shaft-keratin and keratin-associated proteins (KAPs), together with their cross-linked networks-are the underlying reason for its rigid structure.
Sing Ying Wong +2 more
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Two-dimensional gel electrophoresis data for proteomic profiling of Sporothrix yeast cells [PDF]
Data in Brief, 2015 Sporotrichosis is a chronic infection of the skin and subcutaneous tissues of human and other mammals caused by a complex of cryptic dimorphic fungi in the plant-associated order Ophiostomatales.
Anderson Messias Rodrigues +5 more
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Two-dimensional gel electrophoresis data in support of leaf comparative proteomics of two citrus species differing in boron-tolerance [PDF]
Data in Brief, 2015 Here, we provide the data from a comparative proteomics approach used to investigate the response of boron (B)-tolerant ‘Xuegan’ (Citrus sinensis) and B-intolerant ‘Sour pummelo’ (Citrus grandis) leaves to B-toxicity.
Wen Sang +5 more
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Two-dimensional gel electrophoresis in bacterial proteomics. [PDF]
Protein Cell, 2012 Two-dimensional gel electrophoresis (2-DE) is a gel-based technique widely used for analyzing the protein composition of biological samples. It is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal biophysical separation techniques: isoelectric ...
Curreem SO, Watt RM, Lau SK, Woo PC.
europepmc +6 more sources
Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones
Tecnología en Marcha, 2022 A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses.
José Arturo Molina-Mora +3 more
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What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?
Proteomes, 2020 Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus +2 more
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A Novel Spot-Enhancement Anisotropic Diffusion Method for the Improvement of Segmentation in Two-dimensional Gel Electrophoresis Images, Based on the Watershed Transform Algorithm [PDF]
Iranian Journal of Medical Physics, 2015 Introduction Two-dimensional gel electrophoresis (2DGE) is a powerful technique in proteomics for protein separation. In this technique, spot segmentation is an essential stage, which can be challenging due to problems such as overlapping spots, streaks,
Sina Shamekhi +3 more
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Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
, 1982 The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő +3 more
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