Results 31 to 40 of about 210,987 (303)

Automatic selection of two-dimensional electrophoresis gel with least geometric distortions

Mokslas: Lietuvos Ateitis, 2010
The paper presents an algorithm for automated selection of the highest quality two-dimensional gel electrophoresis image. The quality criterion is the amount of vertical geometric distortions of the gel.
Dalius Matuzevičius
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold, An, Andersen, Anderson, Anderson, Anderson, Anderson, Anderson, Appel, Artoni, Baczek, Baczek, Bell, Berggren, Bjellqvist, Bjellqvist, Bonner, Braun, Bravo, Bravo, Bravo, Bravo, Brown, Bunai, Bunger, Burre, Bury, Caglio, Caglio, Camacho-Carvajal, Cantin, Cargile, Cargile, Cargile, Castellanos-Serra, Cejka, Chen, Chevalier, Chevallet, Chiari, Chiari, Chick, Claverol, Cleveland, Cohen, Colinge, Corbett, Coughenour, Courchesne, Cox, Daban, Daban, Davis, Domon, Dukan, Duncan, Egen, Eng, Eravci, Essader, Fernandez-Patron, Figeys, Fritz, Garrels, Garrels, Gelfi, Gelfi, Giometti, Gordon, Gordon, Gorg, Gorg, Gorg, Groleau, Gronow, Han, Hardison, Hartinger, Heller, Henrich, Henzel, Hisabori, Hoffert, Hoffmann, Horowitz, Horth, Hunzinger, James, Johnson, Johnston, Jorgensen, Jovin, Jovin, Kasicka, Keller, Kobayashi, Krijgsveld, Krokhin, Kuroda, Kuster, Kyte, Laemmli, Lambert, Langen, Lasonder, Lengqvist, Liang, Link, Liu, Locke, Loo, Lopez, Lu, Lu, Lu, Luche, Lui, Luo, Luque-Garcia, Lyubimova, Macfarlane, Macfarlane, MacGillivray, Mackintosh, Malmstrom, Malone, Matsudaira, Mazzeo, Mets, Moritz, Nakamura, Navarre, Neuhoff, Nielsen, Novakova, O'Farrell, O'Farrell, Ogorzalek Loo, Orrick, Ortiz, Oses-Prieto, Pappin, Patton, Peng, Perkins, Petritis, Phelps, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Radola, Radola, Rais, Reid, Reynolds, Richert, Rigaut, Righetti, Righetti, Righetti, Righetti, Righetti, Ros, Rosenfeld, Sanchez, Santoni, Schagger, Schagger, Scherl, Schirle, Schluesener, Schmitt, Sevinsky, Sevinsky, Shang, Shapiro, Shen, Shimura, Shimura, Siemankowski, SimoAlfonso, Simpson, Steinberg, Steinberg, Switzer, Szponarski, Szponarski, Tabb, Tan, Tarroux, Tastet, Towbin, Tsugita, Tuszynski, Unlu, van Oostveen, Vonguyen, Voris, Wang, Wang, Washburn, Weber, Wen, West, White, Wilkins, Williams, Wiltfang, Winkler, Wise, Witze, Xiao, Xie, Xie, Yamaguchi, Yamaguchi, Yata, Yates, Young, Yu, Yuan, Zahedi, Zewert, Zischka, Zuo   +225 more
core   +4 more sources

A Routine ‘Top-Down’ Approach to Analysis of the Human Serum Proteome

Proteomes, 2017
Serum provides a rich source of potential biomarker proteoforms. One of the major obstacles in analysing serum proteomes is detecting lower abundance proteins owing to the presence of hyper-abundant species (e.g., serum albumin and immunoglobulins ...
Arlene M. D’Silva, Jon A. Hyett, Jens R. Coorssen   +2 more
doaj   +1 more source

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Biological Procedures Online, 2009
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Mandal Nakul, Heegaard Steffen, Prause Jan, Honoré Bent, Vorum Henrik   +4 more
doaj   +1 more source

Image Analysis Workflow for 2-D Electrophoresis Gels Based on ImageJ [PDF]

, 2011
A number of commercial software packages are currently available to perform digital two-dimensional electrophoresis (2D-GE) gel analysis. However, both the high cost of the commercial packages and the unavailability of a standard data analysis workflow ...
Abrescia, P., Bucci, Em, Maresca, B., Natale, Massimo   +3 more
core   +1 more source

Application of Fluorescence Two-Dimensional Difference In-Gel Electrophoresis as a Proteomic Biomarker Discovery Tool in Muscular Dystrophy Research

Biology, 2013
In this article, we illustrate the application of difference in-gel electrophoresis for the proteomic analysis of dystrophic skeletal muscle. The mdx diaphragm was used as a tissue model of dystrophinopathy.
Steven Carberry, Margit Zweyer, Dieter Swandulla, Kay Ohlendieck   +3 more
doaj   +1 more source

Mitochondrial ribosome assembly in Neurospora. Structural analysis of mature and partially assembled ribosomal subunits by equilibrium centrifugation in CsCl gradients [PDF]

, 1982
In Neurospora, one protein associated with the mitochondrial small ribosomal subunit (S-5, Mr 52,000) is synthesized intramitochondrially and is assumed to be encoded by mtDNA.
Lambowitz, Alan M., LaPolla, Robert J.
core   +1 more source

Comparative 2-DE protein analysis in a 3-D geometry gel

BioTechniques, 2007
Two-dimensional gel electrophoresis (2-DE) separation has not been considered suitable for large-scale comparative protein expression studies due to its limited throughput.
Robert Ventzki, Sabrina Rüggeberg, Stefan Leicht, Thomas Franz, Josef Stegemann   +4 more
doaj   +1 more source

Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry [PDF]

, 2008
Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features,
Berggren, Chevalier, Chevallet, Chevallet, Choi, Gharahdaghi, Heukeshoven, Heukeshoven, Lamanda, Luche, Mackintosh, Neuhoff, Osés-Prieto, Perkins, Porro, Rabilloud, Rabilloud, Rabilloud, Rabilloud, Richert, Shevchenko, Steinberg, Suji, Tastet, Ulrich, Unlu, Winkler   +26 more
core   +4 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source